11. HPLC

HPLC

  • Extraction procedure
    • Saponification to cleave mycolic acids
    • Extraction into chloroform

    Mycolic acids converted to UV absorbing p-bromophenacyl esters

    HPLC Lab Setup

  • Interpretation of patterns
    • Late emerging single-cluster peak pattern
      Late emerging single-cluster peak pattern
    • Widely separated double-peak clusters
      Widely separated double-peak clusters
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  • Examples of rapid growers
    • M. fortuitum complex
      M. fortuitum complex
    • M. chelonae complex
      M. chelonae complex