News
The FACS Core has upgraded the 488nm blue laser on the LSRII Green (TAC613) and the 488nm and 640nm lasers on the LSRII (TAC 6).
Both machines previously had 20mw 488nm lasers; they now are equipped with 50mw 488nm lasers. The LSRII has also been upgraded to a 40mw 640nm laser instead of a 20mw line. Users will notice increased sensitivity and resolution of populations with these new lasers. Users will also notice slight differences in FSC and SSC voltages on these machines.
Questions about laser power or filters can be directed to: Ewa.Menet@yale.edu (5-7958) or Geoffrey.Lyon@yale.edu (7-5959) as there are slight difference between each machine. Users can also view the lasers and filters at: http://medicine.yale.edu/labmed/cellsorter/instrumentation/analysis/index.aspx
The FACS Facility would like to announce the arrival of our newest cell sorter the 300 George Street SORP FACSAria II.
The 300 George St. Aria is located on the third floor in room 345A. The Aria is an 18-parameter, 5-laser system which includes:
100mw 355nm UV laser with 2 PMTs
200mw 405nm Violet laser with 6 PMTs
200mw 488nm Blue laser with 3 PMTs
200mw 532nm Green laser with 4 PMTs
150mw 637nm Red laser with 3 PMTs .
The high powered lasers of the George Street Aria will provide excellent excitation and separation for most commonly used fluorochromes. It is also capable of sorting with 70, 85, 100, and 130 micron nozzles. The UV laser will allow users to perform side population (SP) detection. The George St. Aria is equipped with FACSDiva 7, which allows for index plate sorting. It also has an aerosol management system and is capable of conducting BSL-1 and BSL-2 sorts.
The George St. sort operator is Zhao Zhao. He will begin sorting at George St. on March 11th. If you would like to schedule a sort on the George St. Aria please contact Zhao at zhao.zhao@yale.edu. Users who would like to use the George St. Aria that do not already have access to George St. room 345A will have to provide their netID
Both machines previously had 20mw 488nm lasers; they now are equipped with 50mw 488nm lasers. The LSRII has also been upgraded to a 40mw 640nm laser instead of a 20mw line. Users will notice increased sensitivity and resolution of populations with these new lasers. Users will also notice slight differences in FSC and SSC voltages on these machines.
Questions about laser power or filters can be directed to: Ewa.Menet@yale.edu (5-7958) or Geoffrey.Lyon@yale.edu (7-5959) as there are slight difference between each machine. Users can also view the lasers and filters at: http://medicine.yale.edu/labmed/cellsorter/instrumentation/analysis/index.aspx
The FACS Facility would like to announce the arrival of our newest cell sorter the 300 George Street SORP FACSAria II.
The 300 George St. Aria is located on the third floor in room 345A. The Aria is an 18-parameter, 5-laser system which includes:
100mw 355nm UV laser with 2 PMTs
200mw 405nm Violet laser with 6 PMTs
200mw 488nm Blue laser with 3 PMTs
200mw 532nm Green laser with 4 PMTs
150mw 637nm Red laser with 3 PMTs .
The high powered lasers of the George Street Aria will provide excellent excitation and separation for most commonly used fluorochromes. It is also capable of sorting with 70, 85, 100, and 130 micron nozzles. The UV laser will allow users to perform side population (SP) detection. The George St. Aria is equipped with FACSDiva 7, which allows for index plate sorting. It also has an aerosol management system and is capable of conducting BSL-1 and BSL-2 sorts.
The George St. sort operator is Zhao Zhao. He will begin sorting at George St. on March 11th. If you would like to schedule a sort on the George St. Aria please contact Zhao at zhao.zhao@yale.edu. Users who would like to use the George St. Aria that do not already have access to George St. room 345A will have to provide their netID
to gain keycard access to the room.
With Zhao taking over the George St. Aria, Tom Taylor will be the new TAC Aria operator. If you would like to schedule a sort on the TAC Aria please contact Tom at thomas.taylor@yale.edu.
If you have any questions about the new sorter please contact Geoff Lyon, or Zhao Zhao.
With Zhao taking over the George St. Aria, Tom Taylor will be the new TAC Aria operator. If you would like to schedule a sort on the TAC Aria please contact Tom at thomas.taylor@yale.edu.
If you have any questions about the new sorter please contact Geoff Lyon, or Zhao Zhao.
Mario Roederer, Ph.D., Senior Investigator at ImmunoTechnology Section of Vaccine Research Center, NIAID, NIH recently discovered that one type of "bullet" tube they use for staining leads to large increases in autofluorescence, primarily in the "V450" channel (i.e., excited by near-UV laser, 405 nm, and emitting in the 450 nm range). This is the detector you would use for Pacific Blue, ViViD, or Brilliant Violet 450 dyes. Interestingly, the problem only occurs in the tubes that have been sterilized by gamma-irradiation -- the unsterilized tubes are fine. The tubes are polypropylene, so it's quite possible that this issue may extend to other tubes that are similarly sterilized. The specific tubes come from USA Scientific. The "problem tubes" are catalog #1412-1410; the acceptable tubes are catalog #1412-0400. The sterilization process must be leading to the generation of a UV-excitable dye which leaches into the cells. The longer you incubate cells in the "problem" tubes, the brighter they become. Curiously, this doesn't always happen uniformly -- sometimes half the cells become bright and half do not. Perhaps it requires close proximity of the cell to the plastic. This heterogeneity is insidious -- it looks like you are generating new subsets of cells (rather than having a staining artefact).
Stratedigm analyzers: how you can export an experiment or any part of it, including Instrument Settings, and then load it on any of the other Stratedigm machines. Please see the detailed instructions.
Ashraf Khalil, Associate Research Scientist in Laboratory Medicine, uses Amnis Imaging cytometer in his work. His very recent publication in Science:
http://www.sciencemag.org/content/early/2012/05/02/science.1213368.full#aff-1
http://www.sciencemag.org/content/early/2012/05/02/science.1213368.full#aff-1
The FACS Core will change our online scheduling program for all reservations from May 1st onward. The new FACS Core scheduling website will bewww.yaleflowscheduling.com. Users can log in to www.yaleflowscheduling.com by using their current schedulebook.com e-mail and password.
Users who wish to sign-up for analyzer usage from now through April 30th should still schedule their time on schedulebook.com. The schedulebook.com calendar will be blocked on May 1st and beyond.
Please see the PowerPoint Presentation explaining how to navigate www.yaleflowscheduling.com. In general it will resemble Schedulebook in look and function.
If you have questions please contact Geoff Lyon at geoffrey.lyon@yale.edu or 737-5959.
Users who wish to sign-up for analyzer usage from now through April 30th should still schedule their time on schedulebook.com. The schedulebook.com calendar will be blocked on May 1st and beyond.
Please see the PowerPoint Presentation explaining how to navigate www.yaleflowscheduling.com. In general it will resemble Schedulebook in look and function.
If you have questions please contact Geoff Lyon at geoffrey.lyon@yale.edu or 737-5959.
The LEPH 901B FACS Aria now has state and university approval to sort BSL-3 materials. The LEPH Sorter can sort BSL-1, BSL-2, and BSL-3 cells . The LEPH Aria is equipped with a 407, 488, 532, and 640nm lasers and is contained in a Baker Bio-protect IV hood. It is capable of tube and plate sorting. All new users must have prior approval to conduct BSL-3 research and have room 901B added as a research location with the Connecticut Department of Public Health, through Maryjo Lanzillotta (maryjo.lanzillotta@yale.edu) in OEHS. Researchers interested in sorting BSL-3 material should contact Geoff Lyon (geoffrey.lyon@yale.edu, 737-6471 or 737-5959) to fill out the required forms to begin BSL-3 sorting.
Our new location at 300 George Street: LSRII and Calibur are now located on the second floor in room 2320H. Also the TAC6 STD-8 (Stratedigm) has been relocated to 2320H. The TAC6 STD-8 has been renamed in Schedulebook as STD-8 George. All FACS Core users have 24/7 access to 2320H George Street. The room is located at the end of the north hallway on the second floor inside of the 2320 suite door. 2320H is located to the right directly inside the suite door. Please make sure that when you exit 2320H that you exit using original door you entered the suite through. Please do not use the door down the long corridor which is a space occupied by YARC. If you have questions about the facility or move please contact Esther Beaudoin (5-7958, 5-7949, 7-7452, Esther.Beaudoin@yale.edu) or Geoff Lyon (7-5959, Geoffrey.lyon@yale.edu).
The Cell Sorter Core Facility would like to announce our newest cell sorter located in LEPH. The four laser, up to 15 colors BD FACSAria II is available for all types of sorts, but those wishing to use it have to complete these minimal requirements: 1)Have YARC Approval for Animal Handling (or Complete a Medical Surveillance Form, see YARC website) and 2)Complete a tour of LEPH Facility. To sign up for a tour contact Geoff Lyon at geoffrey.lyon@yale.edu
