Beckman Coulter MoFlo

Room: TAC S617
Contact:  Gouzel.Tokmoulina@yale.edu or (203)737-2891

Manufacturer's Website

  • High-Performance Cell Sorter
  • Data acquisition software: Summit
  • Instrument capabilities: Three laser system, up to 8 compensated colors, 10 colors total
  • 8 color experiment example: FITC, PE, PerCP-Cy5.5, PE-Cy7, APC, AlexaFluor 680, APC-Cy7, Texas Red
  • Single cell deposition into slides and various size multiwell plates. Cells can be sorted into 5,15,50 ml, Eppendorf test tubes and small Petri dishes.
  • Desirable concentration of cells: up to 60x10^6, depending on the cell type and amount of material.
  • Limitations: No cells transformed with viruses or human/primate cells can be sorted on this instrument. 

Blue Laser - 488nm

PMT

Filter

Fluorochrome

FL1

530/40 or 513/24

GFP, YFP, FITC, Alexa Fluor 488

FL2

580/30

PE

FL3

670/30 or 670/40

PerCP-Cy5.5, PE-Cy5, 7-AAD, PI, PE-AlexaFluor 610

FL4

740 LP

PE-Cy7

Green laser - 532 nm

PMT

Filter

Fluorochrome

FL8

613/20 or 630/30

mCherry, Texas-Red, Katushka
AlexaFluor 568

FL9

580/23

PE, DsRed, RFP

FL10

740 LP

PE-Cy7

Red laser - 633 nm

PMT

Filter

Fluorochrome

FL5

670/30

APC

FL6

710/40

AlexaFluor 680, AlexaFluor 700

FL7

740 LP

APC-Cy7