Beckman Coulter MoFlo
Room: TAC S617
Contact: Gouzel.Tokmoulina@yale.edu or (203)737-2891
- High-Performance Cell Sorter
- Data acquisition software: Summit
- Instrument capabilities: Three laser system, up to 8 compensated colors, 10 colors total
- 8 color experiment example: FITC, PE, PE-Cy5, PE-Cy7, APC, AlexaFluor 680, APC-Cy7, RFP
- Single cell deposition into slides and various size multiwell plates. Cells can be sorted into 5,15,50 ml, Eppendorf test tubes and small Petri dishes.
- Desirable concentration of cells: up to 60x10^6, depending on the cell type and amount of material.
- Limitations: No cells transformed with viruses or human/primate cells can be sorted on this instrument.
Blue Laser - 488nm
PMT | Filter | Fluorochrome |
FL1 | 530/40 or 513/24 | GFP, YFP, FITC, Alexa Fluor 488 |
FL2 | 580/30 | PE |
FL3 | 670/30 | PerCP-Cy5.5, PE-Cy5, 7-AAD, PI, PE-AlexaFluor 610 |
FL4 | 740 LP | PE-Cy7 |
Green laser - 532 nm
PMT | Filter | Fluorochrome |
FL8 | 613/20 | mCherry, Texas-Red,
|
FL9 | 580/23 | PE, DsRed, RFP |
FL10 | 725/40 | PE-Cy7 |
Red laser - 633 nm
PMT | Filter | Fluorochrome |
FL5 | 670/30 | APC |
FL6 | 710/40 | AlexaFluor 680, AlexaFluor 700 |
FL7 | 740 LP | APC-Cy7 |


