BD FACS Aria in LEPH

Room: 901B at 60 College Street

Contact: (203)737-5959, (203)737-6471, geoffrey.lyon@yale.edu


The LEPH  BD FACS Aria II is contained in a Baker BioProtect IV Biological Safety Cabinet.

It has  has 405nm, 488nm, 532nm, and 640nm lasers for up to 15-colors.

The 405nm is equipped with 5 PMTs, the 488nm – 2 PMTs, 532nm – 5 PMTs, and the 640nm – 3 PMTs.

The LEPH Aria is capable of up to 4-way tube collection, plate deposition, and single cell deposition.

The LEPH Sorter can sort BSL-1, BSL-2, and BSL-3 cells .

 The sorter is available for all types of sorts, but those wishing to use it have to complete these minimal requirements:

1)Have YARC Approval for Animal Handling (or Complete a Medical Surveillance Form, see YARC website)

2)Complete a tour of the LEPH Facility

To sign up for a tour contact Geoff Lyon at geoffrey.lyon@yale.edu

BD Biosciences web site

Violet Laser - 405nm

PMTBandpassLong PassFluorochrome
E    450/50Pacific Blue/Horizon V450
D525/50495LPAmCyan/Horizon V500
C610/20600LPQdot 605
B670/30630LPQdot 655
A710/50685LPQdot 705

Blue Laser - 488nm

PMTBandpassLong PassFluorochrome
B
515/20
505LPFITC
A
710/50
 685LPPerCP-Cy5.5

Green Laser - 532nm

PMTBandpassLong PassFluorochrome
E575/26
PE
D
610/20600LPPE-Texas Red
C660/40640LPPE-Cy5
B
710/50
690LPPE-Cy5.5
A
780/40
740LPPE-Cy7


 

Red Laser - 640nm

PMTBandpassLong PassFluorochrome
C
660/20
APC
B710/50685LPAPC-Cy5.5
A
780/60
 740LPAPC-Cy7