BD FACS Vantage SE
Room: TACS617
Contact:Thomas.Taylor@yale.edu or (203)785-2851
- High speed cell sorter, up to 25 K/sec
- Three lasers, up to eight colors
- Data acquisition software: CellQuest or Diva
- 488 nm : FITC,GFP, PE, PE-Cy5, PE-Cy7, YFP, 7-AAD
- 568 nm: RFP, Texas-Red, Alexa 568
- 633 nm : APC, APC-Cy7, Cy5, Alexa Fluor 680/700
- Example of five colors: FITC, PE, PE-Cy5, APC, APC-Cy7
- Applications: Single cell deposition
- 4 way sorting
- Cell concentration in a sample 20x10^6 cell/ml
- Sample tubes are Falcon 352054
- Collection tubes: 5ml, 15 ml
Blue Laser - 488nm
PMT | Filter | Fluorochrome |
530/30 | GFP,YFP, FITC,Alexa Fluor 488 | |
575/26 | PE | |
670/40 | PerCP-Cy5.5, PE-Cy5.5, 7-AAD, PI | |
780/60 | PE-Cy7 |
Green laser - 568 nm
PMT | Filter | Fluorochrome |
610/20 | RFP, Texas-Red, Alexa 568, mCherry, |
Red laser - 633 nm
PMT | Filter | Fluorochrome |
660/20 | APC | |
710/40 | AlexaFluor 680, AlexaFluor 700 | |
740 LP | APC-Cy7 |


