BD FACS Vantage SE

Room: TACS617
Contact:Thomas.Taylor@yale.edu or (203)785-2851

Manufacturer's Website

  • High speed cell sorter, up to 25 K/sec
  • Three lasers, up to eight colors
  • Data acquisition software: CellQuest or Diva
  • 488 nm : FITC,GFP, PE, PE-Cy5, PE-Cy7, YFP, 7-AAD
  • 568 nm: RFP, Texas-Red, Alexa 568
  • 633 nm : APC, APC-Cy7, Cy5, Alexa Fluor 680/700
  • Example of five colors: FITC, PE, PE-Cy5, APC, APC-Cy7
  • Applications: Single cell deposition
  • 4 way sorting
  • Cell concentration in a sample 20x10^6 cell/ml
  • Sample tubes are Falcon 352054
  • Collection tubes: 5ml, 15 ml

Blue Laser - 488nm

PMT

Filter

Fluorochrome

530/30

GFP,YFP, FITC,Alexa Fluor 488

575/26

PE

670/40

PerCP-Cy5.5, PE-Cy5.5, 7-AAD, PI

780/60

PE-Cy7

Green laser - 568 nm

PMT

Filter

Fluorochrome

610/20

RFP, Texas-Red, Alexa 568,  mCherry,

Red laser - 633 nm

PMT

Filter

Fluorochrome

660/20

APC

710/40

AlexaFluor 680, AlexaFluor 700

740 LP

APC-Cy7