FACS Aria at George St.

BD FACSAria

The 300 George St.  FACSAria is located in room 203B/2320H. The 18-parameter, 5-laser system includes:
100mw 355nm UV laser with 2 PMTs
200mw 405nm Violet laser with 6 PMTs
200mw 488nm Blue laser with 3 PMTs
200mw 532nm Green laser with 4 PMTs
150mw 637nm Red laser with 3 PMTs .
It can handle UV excitation for cell-cycle and “side-population”-based sorting.
FACSAria II is capable of sorting with 70, 85, 100, and 130 micron nozzles and equipped with FACSDiva 7, which allows for index plate sorting. It also has an
aerosol management system and is capable of conducting BSL-1 and BSL-2 sorts. 

Anyone interested in having cells sorted can contact Zhao Zhao or call 203-737-7452.

BD Biosciences web site

UV Laser - 355 nm, 100 mW

PMTBandpassLongpassFluorochrome
A675/50635LPHoechst, Side Population
B450/50Indo-1 (Violet), DAPI, Alexa Fluor 350

Violet Laser - 405 nm, 200 mW

PMT

Bandpass

Long Pass

Fluorochrome

A

780/60

750LP

Qdot 800

B

710/50

695LP

Qdot 705

C

670/30

635LP

Qdot 655

D

610/20

595LP

Qdot 605

E

525/50

505LP

AmCyan, V500, Pacific Orange, Alexa Fluor 430, CFP

F

450/50


Pacific Blue, V450, BFP

Blue Laser -488nm, 200 mW

PMTBandpass
Long Pass

Fluorochrome
A710/40685LP
PerCP-Cy5.5, PI(with PE)
B582/15555LP
PE, PI
C525/50505LP

FITC, AlexaFluor488, CFSE, GFP, YFP


488/10

Green Laser - 532 nm, 200 mW

PMT

Bandpass

Long Pass

Fluorochrome

A

780/60

750LP

PE-Cy7

B

670/30 

635LP

PE-CY5 

C

616/23

565LP

PE-Texas Red, RFP

D

575/26 


DsRed, PE, RFP 

Red Laser - 640nm, 150 mW

PMT     BandpassLong PassFluorochrome
A     780/60750LPAPC-Cy7, Alexa Fluor 750, Alexa Fluor 780
B     730/45685LPAPC-Cy5.5, Alexa Fluor 700
C     670/30APC, Alexa Fluor 647