The 300 George St. FACSAria is located on the third floor in room 345A. The 18-parameter, 5-laser system includes:
100mw 355nm UV laser with 2 PMTs
200mw 405nm Violet laser with 6 PMTs
200mw 488nm Blue laser with 3 PMTs
200mw 532nm Green laser with 4 PMTs
150mw 637nm Red laser with 3 PMTs .
It can handle UV excitation for cell-cycle and “side-population”-based sorting.
FACSAria II is capable of sorting with 70, 85, 100, and 130 micron nozzles and equipped with FACSDiva 7, which allows for index plate sorting. It also has an
aerosol management system and is capable of conducting BSL-1 and BSL-2 sorts.
Anyone interested in having cells sorted can contact Zhao Zhao or call 203-737-8068.
BD Biosciences web site
UV Laser - 355 nm, 100 mW
|A||525/50||Hoechst, Side Population|
|B||450/50||Indo-1 (Violet), DAPI, Alexa Fluor 350
Violet Laser - 405 nm, 200 mW
|E||525/50||AmCyan, V500, Pacific Orange, Alexa Fluor430, CFP
|F||450/50||Pacific Blue, V450, BFP
Blue Laser -488nm, 200 mW
|A||710/50||PerCP-Cy5.5, PI(with PE)
FITC, AlexaFluor488, CFSE, GFP, YFP
Green Laser - 532nm
|C||616/20||PE-Texas Red, RFP|
||DsRed, PE, RFP|
Red Laser - 640nm, 150 mW
|A||780/60||APC-Cy7, Alexa Fluor 750, Alexa Fluor 780|
|B||730/45||APC-Cy5.5, Alexa Fluor 700|
|C||670/30||APC, Alexa Fluor 647|