FACS Aria at George St.

The 300 George St.  FACSAria is located in room 203B/2320H. The 18-parameter, 5-laser system includes:
100mw 355nm UV laser with 2 PMTs
200mw 405nm Violet laser with 6 PMTs
200mw 488nm Blue laser with 3 PMTs
200mw 532nm Green laser with 4 PMTs
150mw 637nm Red laser with 3 PMTs .
It can handle UV excitation for cell-cycle and “side-population”-based sorting.
FACSAria II is capable of sorting with 70, 85, 100, and 130 micron nozzles and equipped with FACSDiva 7, which allows for index plate sorting. It also has an
aerosol management system and is capable of conducting BSL-1 and BSL-2 sorts. 

Anyone interested in having cells sorted can contact Zhao Zhao or call 203-737-7452.

BD Biosciences web site


UV Laser - 355 nm, 100 mW 

PMTBandpassFluorochrome
A525/50Hoechst, Side Population
B450/50Indo-1 (Violet), DAPI, Alexa Fluor 350

Violet Laser - 405 nm, 200 mW

PMTBandpassFluorochrome
A780/60Qdot 800
B710/50Qdot 705
C670/30Qdot 655
D610/20
Qdot 605
E525/50AmCyan, V500, Pacific Orange, Alexa Fluor430, CFP
F450/50Pacific Blue, V450, BFP

Blue Laser -488nm, 200 mW

PMTBandpassFluorochrome
A710/50PerCP-Cy5.5, PI(with PE)
B582/15PE, PI
C515/20

FITC, AlexaFluor488, CFSE, GFP, YFP


Green Laser - 532nm

PMTBandpassFluorochrome
A780/60PE-Cy7

670/30PE-Cy5 
C616/20PE-Texas Red, RFP
D
575/26 
DsRed, PE, RFP

Red Laser - 640nm, 150 mW

PMTBandpassFluorochrome
A780/60APC-Cy7, Alexa Fluor 750, Alexa Fluor 780
B730/45APC-Cy5.5, Alexa Fluor 700
C670/30APC, Alexa Fluor 647