FACS Aria in the Amistad building

Room: 416 at 10 Amistad

Contact : (203)785-2299 

The FACS Aria in Amistad building has 4 lasers (488 nm blue laser, 635 nm red laser, 405 nm violet laser and a 355 nm UV laser). This machine is capable of sorting appropriate human materials, and is equipped with aerosol containment system. It can handle UV excitation for cell-cycle and “side-population”-based sorting. Anyone interested in having cells sorted can contact emptyZuzana.Tobiasova@yale.edu.

BD Biosciences web site

UV Laser - 355nm

PMTBandpassLong PassFluorochrome
A670 LP635 LPSide Population
B450/50 Indo-1 (Violet), DAPI, Alexa Fluor 350, Hoechst

Violet Laser - 407nm

PMTBandpassLong PassFluorochrome
A525/50505LPAlexa Fluor 430, CFP, Am Cyan
B450/40 Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue

Blue Laser - 488nm

PMTBandpassLong PassFluorochrome
A780/60735LPPE-Cy7, PE-Alexa Fluor 750
B670/14635LPPI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA
C610/20600LPPE-Texas Red, PI (with PE and PE-Cy5.5)
D575/25550LPPE, PI (with PerCP)
E530/30505LPFITC, GFP, CFSE, YFP, Alexa Fluor 488, SYBR-14
F488/10 SSC

Red Laser - 633nm

PMTBandpassLong PassFluorochrome
A780/60755LPAPC-Cy7, APC-Alexa 750
B710/50685LPAlexa Fluor 680, Alexa Fluor 700
C660/20 APC, Alexa Fluor 647