Instrumentation |
Cell Sorting |
BD FACSAria at Amistad
FACS Aria in the Amistad building
The FACS Aria in Amistad building has 4 lasers (488 nm blue laser, 635 nm red laser, 405 nm violet laser and a 355 nm UV laser). This machine is capable of sorting appropriate human materials, and is equipped with aerosol containment system. It can handle UV excitation for cell-cycle and “side-population”-based sorting. Anyone interested in having cells sorted can contact Geoffrey.Lyon@yale.edu or Zhao.Zhao@yale.edu.
BD Biosciences web site
UV Laser - 355nm
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 670 LP | 635 LP | Side Population |
| B | 450/50 | | Indo-1 (Violet), DAPI, Alexa Fluor 350, Hoechst |
Violet Laser - 407nm
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 525/50 | 505LP | Alexa Fluor 430, CFP, Am Cyan |
| B | 450/40 | | Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue |
Blue Laser - 488nm
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 735LP | PE-Cy7, PE-Alexa Fluor 750 |
| B | 710/50 | 685LP | PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA |
| C | 610/20 | 600LP | PE-Texas Red, PI (with PE and PE-Cy5.5) |
| D | 575/25 | 550LP | PE, PI (with PerCP) |
| E | 530/30 | 505LP | FITC, GFP, CFSE, YFP, Alexa Fluor 488, SYBR-14 |
| F | 488/10 | | SSC |
Red Laser - 633nm
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 755LP | APC-Cy7, APC-Alexa 750 |
| B | 710/50 | 690LP | Alexa Fluor 680, Alexa Fluor 700 |
| C | 660/20 | | APC, Alexa Fluor 647 |
