Instrumentation

For analysis

There are 8 user-operated analyzers in the TAC Building (LSRII, LSRII Green, LSRII TAC5, two FACSCaliburs, three Stratedigm analyzers), LSRII, Stratedigm and FACSCalibur in Amistad, and at 300 George Street  Room 2320H we have a special order LSRII. The Caliburs can analyze up to 4 colors with 488 nm and 633 nm excitation lasers. Stratedigm STD-8 and STD-13 are capable of analyzing 8 and 13 colors. LSRII and LSRII Green have a capability of 12 colors. Stratedigm STD-13, STD-13+L, BD FACSCalibur S, LSRII and LSRII Green are located in room TAC S613; LSRII TAC5, Calibur H, STD-8 TAC5- in room S533. Machines are checked daily for optimal performance. Ewa Menet at TAC is available during working hours to troubleshoot. Internet sign up for analysis time is available from any computer via the internet for users after completing the training.

Bio Rad Bio-Plex System (room S522) is a compact, simple, highly flexible platform for a wide range of bead-based assays. It combines the concept of flow cytometry with dual red and green lasers for simultaneous bead and reporter detection with up to 100 uniquely identifiable fluorescently labeled microspheres. This combination enables bio-molecule analysis in a highly multiplexed format. Individual microspheres can be complexed with any protein, peptide or nucleic acid. For training please contact Ewa.Menet@yale.edu.

Amnis Imagestream-X imaging flow cytometer (room S633)- provides users with the ability to gain detailed images of a large number of cells in a relatively short period of time and with the opportunity to perform a range of novel applications including co-localization, internalization, stem cell differentiation, and cell-cell interactions. For training please contact Ewa.Menet@yale.edu

For cell sorting

Beckman Coulter MoFlo and Sony Reflection located in S617 and FACSAria in LEPH 901B are operated by staff members. FACSArias in TAC S633 and Amistad are operated by staff or can be user-operated after completing the training.

Cells can be sorted into 5 or 15 ml tubes or into various plates as single or multiple cells per well or onto microscopic slides for analysis. In addition, all sorters are capable of simultaneous 4–way sorting. All sorters except MoFlo are also equipped for sorting live human, primate or other potentially biohazardous cells.

Users of the Sony Reflection can take advantage of using two sort heads and a lower charge for the second head to be running.