BD LSRII TAC-5th floor (TAC S533)
Contact : Ewa.Menet@yale.edu or call 203-785-7958
Violet Laser - 405nm 100 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 530/30 | 505LP | Alexa Fluor 430, CFP, Am Cyan |
B | 450/50 | Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue |
Blue Laser - 488nm 50 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/60 | 750LP | PE-Cy7, Alexa 750 PE |
B | 670/14 | 685LP | PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5 |
C | 610/20 | 600LP | PE-Texas Red, PI (with PE and PE-Cy5.5) |
576/26 | PE, PI (with PerCP), Pyronin | ||
D | 530/30 | 505LP | FITC, GFP, CFSE, YFP, Alexa Fluor 488 |
E | 488/10 | SSC |
Red Laser - 640nm 40 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/60 | 735LP | APC-Cy7, APC-Alexa Fluor 750 |
B | 735/45 | 685LP | Alexa Fluor 680, Alexa Fluor 700 |
C | 670/30 | APC, Alexa Fluor 647 |
*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.
