BD LSRII TAC-5th floor (TAC S533)

Contact : Ewa.Menet@yale.edu or call 203-785-7958

Violet Laser - 405nm    100 mW

PMT

Bandpass

Long Pass

Fluorochrome

A

530/30

505LP

Alexa Fluor 430, CFP, Am Cyan

B

450/50

Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue

Blue Laser - 488nm    50 mW

PMT

Bandpass

Long Pass

Fluorochrome

A

780/60

750LP

PE-Cy7, Alexa 750 PE

B

670/14

685LP

PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5

C

610/20

600LP

PE-Texas Red, PI (with PE and PE-Cy5.5)

576/26

PE, PI (with PerCP), Pyronin

D

530/30

505LP

FITC, GFP, CFSE, YFP, Alexa Fluor 488

E

488/10

SSC

Red Laser - 640nm    40 mW

PMT

Bandpass

Long Pass

Fluorochrome

A

780/60

735LP

APC-Cy7, APC-Alexa Fluor 750

B

735/45

685LP

Alexa Fluor 680, Alexa Fluor 700

C

670/30

APC, Alexa Fluor 647

*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.