BD LSR II ( TAC S613 )

Contact: Ewa.Menet@yale.edu, Christine.Cote@yale.edu or call 203-785-7958

  • Multilaser multiparameter analysis cytometer
  • Four lasers, 12 colors
  • Digital data collection using FACS DIVA software
  • Automatic compensation (compensation controls are still needed)
  • 355 nm (UV): Indo-1, DAPI, Alexa Fluor 350
  • 405 nm (Violet): CFP, Alexa Fluor 430, Pacific Blue, Marina Blue, Alexa Fluor 405
  • 488 nm (Blue): FITC, PE, PerCP, Pe-Cy5 (aka CyChrome), PI, PerCp-Cy5.5, PE-Cy7, GFP, PE-Texas Red
  • 633 nm (Red): APC, APC-Cy7, Alexa Fluor 647, Alexa 680
  • Availability of UV gives a capability to perform Ca 2+ flux experiments.
  • Unlike a FACSCalibur, it is possible to use PE-Cy5 and APC together.

UV Laser - 355nm     20 mW

PMTBandpassLong PassFluorochrome
A   740/35690LPBUV737
B
530/30505LP Fura-2, Am-Cyan
B440/40 DAPI, Alexa Fluor 350

**Additional filters are available for Calcium Flux and Side Population detection. Please see the staff about filter swaps.

Violet Laser - 405nm   50 mW

PMTBandpassLong PassFluorochrome
A710/50690LPBV711
B610/20595LPBV605, Alexa Fluor 605, QDot605
C525/50505LPAlexa Fluor 430, CFP, Am Cyan
D440/40 Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP,
Marina Blue, BV421

Blue Laser - 488nm     50 mW

PMTBandpassLong PassFluorochrome
A780/60735LPPE-Cy7, Alexa 750 PE
B670/14635LPPI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA
C610/20600LPPE-Texas Red, PI (with PE and PE-Cy5.5)
D576/26550LPPE, PI (with PerCP), Pyronin
E530/30505LPFITC, GFP, CFSE, YFP, Alexa Fluor 488
F488/10 SSC

Red Laser - 640nm    40 mW

PMTBandpassLong PassFluorochrome
A780/60735LPAPC-Cy7, APC-Alexa Fluor 750
B710/25685LPAlexa Fluor 680, Alexa Fluor 700
C660/20 APC, Alexa Fluor 647

*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.