BD LSRII Green (TAC S613)

Contact: Ewa.Menet@yale.edu or call 203-785-7958

Please note:

on this cytometer APC-Cy7 and PE-Cy7 should not be used together.

Violet Laser - 405nm    20 mW

PMTBandpassLong PassFluorochrome
A710/50690LPBV711
B610/20595LPBV605, Alexa Fluor 605, QDot605
C525/50505LPAlexa Fluor 430, CFP, Am Cyan
D450/50Cascad Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, CFP, BV421

Blue Laser - 488nm    50 mW

PMTBandpassLong PassFluorochrome
A670/14635LPPerCP
B525/50505LPFITC, GFP, PKH67, CFSE, Alexa Fluor 488
C488/10SSC

Green Laser - 532nm    150 mW

PMTBandpassLong PassFluorochrome
A780/60735LPPE-Cy7
B710/50685LpPI, PE-Cy5.5
C660/20630LPPE-Cy5
D610/20600LPAlexa Fluor 594, PE-Texas Red, Alexa Fluor 568, PI, Rhodamine-B, PI (with PE)
E575/26PE, DsRed, RFP, Pyronin-Y, PKH26

Red Laser - 640 nm    40 mW

PMTBandpassLong PassFluorochrome
A780/60755LPAPC-Cy7, APC-Alexa Fluor 750
B710/50690LPAlexa Fluor 680, Alexa Fluor 700
C660/20APC, Alexa Fluor 647

*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.