BD LSRII Green (TAC S613)

Contact: Chrisine.Cote@yale.edu, Ewa.Menet@yale.edu or call 203-785-7958

Please note:

on this cytometer APC-Cy7 and PE-Cy7 should not be used together.

Violet Laser - 405nm    20 mW

PMT Bandpass Long Pass Fluorochrome
A 710/50 690LP BV711
B 610/20 595LP BV605, Alexa Fluor 605, QDot605
C 525/50 505LP Alexa Fluor 430, CFP, Am Cyan
D 450/50   Cascad Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, CFP, BV421

Blue Laser - 488nm    50 mW

PMT Bandpass Long Pass Fluorochrome
A 670/14 635LP PerCP
B 525/50 505LP FITC, GFP, PKH67, CFSE, Alexa Fluor 488
C 488/10   SSC

Green Laser - 532nm    150 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 735LP PE-Cy7
B 710/50 685Lp PI, PE-Cy5.5
C 660/20 630LP PE-Cy5
D 610/20 600LP Alexa Fluor 594, PE-Texas Red, Alexa Fluor 568, PI, Rhodamine-B, PI (with PE)
E 575/26   PE, DsRed, RFP, Pyronin-Y, PKH26

Red Laser - 640 nm    40 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 755LP APC-Cy7, APC-Alexa Fluor 750
B 710/50 690LP Alexa Fluor 680, Alexa Fluor 700
C 660/20   APC, Alexa Fluor 647

*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.