Instrumentation |
FACS Analysis |
BD LSRII at George St
BD LSRII at George Street
Please note:
On this cytometer APC-Cy7 and PE-Cy7 should not be used together.
Violet Laser - 405 nm 100 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 605/40 | 595 | Qdot 605 |
| B | 525/50 | 505 | AmCyan, CFP |
| C | 450/50 | | Pacific blue, Alexa Fluor 405, CFP, BFP, Cascade blue, Sky blue |
Blue Laser - 488 nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 685/35 | 670 | PerCP |
| B | 525/50 | 505 | FITC, GFP, Alexa 488, CFSE, YFP |
| C | 488/10 | | SSC |
Green Laser - 532 nm 150 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 750 | PE-Cy7 |
| B | 710/50 | 685 | PE-Cy5.5 |
| C | 670/30 | 635 | PE-Cy5 |
| D | 610/20 | 600 | PE-Texas Red, PI, AlexaFluor 594, AlexaFluor 568, Rhodamine-B |
| E | 582/15 | | PE, DS Red, RFP, Alexa Fluor 532 |
Red Laser - 640 nm 70 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 750 | APC-Cy7, Alexa Fluor 750 |
| B | 730/40 | 685 | Alexa Fluor 700, Alexa Fluor 680 |
| C | 670/30 | | APC, Alexa Fluor 647 |
