BD LSRII at George Street

Please note:
On this cytometer APC-Cy7 and PE-Cy7 should not be used together.


Violet Laser - 405 nm  100 mW

PMT Bandpass Long Pass Fluorochrome

A 710/50 690 BV711
B 610/20 595 BV605, Qdot 605
C 525/50 505 AmCyan, CFP
D 450/50 Pacific blue, Alexa Fluor 405, CFP, BFP, Cascade blue, Sky blue, BV421

Blue Laser - 488 nm  50 mW

PMT Bandpass Long Pass Fluorochrome
A 685/35 670 PerCP
B 525/50 505 FITC, GFP, Alexa 488, CFSE, YFP
C 488/10 SSC

Green Laser - 532 nm  150 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 750 PE-Cy7
B 710/50 685 PE-Cy5.5
C 670/30 635 PE-Cy5
D 610/20 600 PE-Texas Red, PI, AlexaFluor 594, AlexaFluor 568, Rhodamine-B
E 582/15 PE, DS Red, RFP, Alexa Fluor 532

Red Laser - 640 nm   70 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 750 APC-Cy7, Alexa Fluor 750
B 730/40 685 Alexa Fluor 700, Alexa Fluor 680
C 670/30 APC, Alexa Fluor 647