BD FACSCalibur

  • 4 color analysis cytometer
  • Recommended fluorochromes:
    • FITC and Alexa Fluor 488 (FL1 channel)
    • PE (FL2)
    • PerCP, PE-Cy5, PerCP-Cy5.5 and PE-Cy7 for FL3 channel
    • APC and Alexa 647 for FL4

Please note that Pe-Cy5 and APC should not be used simultaneously on FACSCaliburs.


The facility has two FACSCaliburs in TAC: FACSCalibur S in TAC S613 and FACSCalibur H in TAC S533.
The facility at George St. has one FACSCalibur in room 345A and Amistad has one Calibur in room 105.