Instrumentation |
FACS Analysis |
BD LSRII at Amistad
BD LSRII at Amistad
Please note:
on this cytometer APC-Cy7 and PE-Cy7 should not be used together.UV Laser - 355nm 60 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 525/50 | 505 LP | Indo-1 (Blue), Hoechst Red, Fura-2, Am-Cyan |
| B | 450/50 | | Indo-1 (Violet), DAPI, Alexa Fluor 350, Hoechst |
Violet Laser - 405nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 525/50 | 505LP | Alexa Fluor 430, CFP, Am Cyan |
| B | 450/50 | | Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, CFP |
Blue Laser - 488nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 670/14 | 635LP | PerCP |
| B | 525/50 | 505LP | FITC, GFP, PKH67, CFSE, Alexa Fluor 488 |
| C | 488/10 | | SSC |
Green Laser - 532nm 150 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 735LP | PE-Cy7, |
| B | 710/50 | 685Lp | PI, PE-Cy5.5, |
| C | 660/20 | 630LP | PE-Cy5 |
| D | 610/20 | 600LP | Alexa Fluor 594, PE-Texas Red, Alexa Fluor 568, PI, Rhodamine-B, PI (with PE and PE-Cy5.5) |
| E | 575/26 | | PE, DsRed, RFP, Pyronin-Y, PKH26, |
Red Laser - 640nm 70 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 755LP | APC-Cy7, APC-Alexa Fluor 750 |
| B | 710/50 | 690LP | Alexa Fluor 680, Alexa Fluor 700 |
| C | 660/20 | | APC, Alexa Fluor 647 |
*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.
