BD LSRII at Amistad

BD LSRII at Amistad

UV Laser - 355nm    60 mW

PMT Bandpass Long Pass Fluorochrome
A 740/35 690LP BUV737
B 450/50
410LP DAPI, Alexa Fluor 350
C 379/28   BUV395

**DAPI and BUV395 Should not be used together.

Additional filters are necessary for Calcium Flux and Side Population detection, please see the staff about filters swaps. 

Violet Laser - 405nm   50 mW

PMT Bandpass Long Pass Fluorochrome
A 710/50 690LP BV711
B 610/20 595LP BV605, Alexa Fluor 605, QDot605
C 525/50 505LP Alexa Fluor 430, CFP, Am Cyan, Pacific Orange
D 450/50   Cascade Blue, Pacific Blue, Sky Blue, Alexa Fluor 405, BFP, CFP, Hoechst

Blue Laser - 488nm   50 mW

PMT Bandpass Long Pass Fluorochrome
A 685/35 630LP PerCP, EMA, PercP-Cy5.5
B 525/50 505LP FITC, GFP, PKH67, CFSE, Alexa Fluor 488, YFP*
C 488/10   SSC

*YFP-if detecting YFP and GFP ask for filters change on A and B PMT's

Green Laser - 532nm   150 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 735LP PE-Cy7, PE-alexa750
B 710/50 685Lp PE-Cy5.5
C 675/20 630LP PE-Cy5, PE-Alexa647
D 610/20 600LP Alexa Fluor 594*, PE-Texas Red, Alexa Fluor 568*, PI, Rhodamine-B, PI
E 575/26   PE, DsRed, RFP, Pyronin-Y, PKH26

*Alexa Fluor 594 only 25% Ex., *Alexa Fluor 568 only 34% Ex.

Red Laser - 640nm  70 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 755LP APC-Cy7, Alexa Fluor 750*
B 710/50 690LP Alexa Fluor 680, Alexa Fluor 700
C 660/20   APC, Alexa Fluor 647

Alexa Fluor 750 only 18% Ex.