BD LSRII at Amistad

Please note:
on this cytometer APC-Cy7 and PE-Cy7 should not be used together.

UV Laser - 355nm    60 mW

PMTBandpassLong PassFluorochrome
A525/50505 LPIndo-1 (Blue), Hoechst Red, Fura-2, Am-Cyan
B450/50 Indo-1 (Violet), DAPI, Alexa Fluor 350, Hoechst

Violet Laser - 405nm   50 mW

PMTBandpassLong PassFluorochrome
A525/50505LPAlexa Fluor 430, CFP, Am Cyan
B450/50 Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, CFP

Blue Laser - 488nm   50 mW

PMTBandpassLong PassFluorochrome
A670/14635LPPerCP
B525/50505LPFITC, GFP, PKH67, CFSE, Alexa Fluor 488
C488/10 SSC

Green Laser - 532nm   150 mW

PMTBandpassLong PassFluorochrome
A780/60735LPPE-Cy7,
B710/50685LpPI, PE-Cy5.5,
C660/20630LPPE-Cy5
D610/20600LPAlexa Fluor 594, PE-Texas Red, Alexa Fluor 568, PI, Rhodamine-B, PI (with PE and PE-Cy5.5)
E575/26 PE, DsRed, RFP, Pyronin-Y, PKH26,

Red Laser - 640nm  70 mW

PMTBandpassLong PassFluorochrome
A780/60755LPAPC-Cy7, APC-Alexa Fluor 750
B710/50690LPAlexa Fluor 680, Alexa Fluor 700
C660/20 APC, Alexa Fluor 647

*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.