Amnis Imagestream-X imaging flow cytometer
Room: S633
Contact: Ewa.Menet@yale.edu or (203)785-7958
Amnis Imagestream-X imaging flow cytometer provides users with the ability to gain detailed images of a large number of cells in a relatively short period of time and with the opportunity to perform a range of novel applications including co-localization, internalization, stem cell differentiation, and cell-cell interactions.
ImageStreamX produces up to 12 high resolution images of each cell directly in flow, at rates exceeding 1,000 cells per second, and with the fluorescence sensitivity of conventional flow cytometers. These capabilities allow you to quantitate cellular morphology and the intensity and location of fluorescent probes on, in, or between cells, even in rare sub-populations and highly heterogeneous samples.
With the ImageStreamX you can:
- Image cells directly in suspension with the resolution of a 40X microscope and the fluorescence sensitivity of flow cytometers
- Analyze highly heterogeneous samples and rare cell sub-populations at speeds exceeding 1,000 cells per second
- Perform phenotypic and functional studies at the same time using up to five lasers and 12 images per cell
- Quantitate virtually anything you can see using the IDEAS® software package’s numerous pre-defined fluorescence and morphologic parameters
ImageStreamX Optics
ImageStreamX has 4 lasers (488 nm blue laser, 561 nm green laser, 635 nm red laser, 405 nm violet laser and a 785 nm laser used by SSC). The output of each laser can be independently adjusted from 10 to 100 percent of maximum laser power for greater experimental flexibility and optimal results.
ImageStreamX Fluorochrome Chart – 2 camera STD
Camera 1
Excitation Laser (nm) | ||||||
Ch | Band(nm) | 405 | 488 | 561 | 658 | 785 |
1 | 430-480 | BRIGHTFIELD | ||||
2 | 480-560 | FITC, AF488, GFP, YFP, DyLight488, PKH67, Syto 13, Spectrum Green, Lyso TrackerGreen, Mito TrackerGreen, QD525* | ||||
3 | 560-595 | PE, PKH26, Cy3, AF555, DSRed | PE, Cy3, AF546, AF555, DyLight549, DyLight594*, PKH26, DSRed, SpectrumOrange, Mito TrackerOrange | |||
4 | 595-660 | PE-TexRed*, ECD*, PE-AF610*, 7AAD*, PI*, RFP, QD625*, eFluor625* | AF568*, AF594*, AF610*, DyLight594*, PE-TexRed*, ECD*, TexRed*, PE-AF610*, RFP, mCherry*, 7AAD*, PI* | |||
5 | 660-745 | PE-Cy5*, PE-AF647*, PerCP*, Per-CP-Cy5.5*, DRAQ5*, QD705*, eFluor650* | PE-Cy5*, PE-AF647*, DRAQ5* | |||
6 | 745-800 | PE-Cy7*, PE-AF750*, QD800 | PE-Cy7*, PE-AF750*, | SSC | ||
Camera 2
Excitation Laser (nm) | ||||||
Ch | Band(nm) | 405 | 488 | 561 | 658 | 785 |
7 | 430-505 | DAPI, Hoechst, PacBlue, CascadeBlue, AF405, eFluor405, DyLight405, CFP, LIVE/DEAD Violet | ||||
8 | 505-570 | PacOrange, Cascade Yellow, AF430, QD525* | ||||
9 | 570-595 | BRIGHTFIELD | ||||
10 | 595-660 | QD625*, eFluor625* | ||||
11 | 660-745 | QD705*, eFluor650* | AF647, AF660, AF680, DRAQ5*, APC, Cy5, DyLight649, DyLight680, PE-AF647*, PE-Cy5*, PerCP*, PerCP-Cy5.5* | |||
12 | 745-800 | QD800* | APC-Cy7, APC-AF750, APC-eFluor750, Cy7, AF750, DyLight750, eFluor750, PE-Cy7*, PE-AF750* | |||
Dyes with an* are excited by one laser directed to camera 1 and another laser directed to camera 2. The channel that the dye will appear brightest in depends on the relative laser power used. Recommended dyes (based on excitation and detection channels) are in boldface. QD565 and QD585 are not included because their primary fluorescence appears in the Ch9 Brightfield.
