The Yale FACS Core is happy to announce the arrival of our new FACS Aria which we have designated TAC Aria-A, for aseptic.
This sorter is intended to be free of pathogen-associated molecular pattern (PAMPs), for cell sorts with a minimal exposure to microbial components.
In order to maintain such a high level of cleanliness certain materials will be prohibited from being sorted on the TAC Aria-A.
TAC Aria-A will NOT sort:
- Any microbial samples
- Cells derived from the gut with high bacterial content (intestine, pelvic lavage,...)
- Skin cells
- Cells from the esophagus and upper lung
- Mario Roederer, Ph.D., Senior Investigator at ImmunoTechnology Section of Vaccine Research Center, NIAID, NIH discovered that one type of "bullet" tube they use for staining leads to large increases in autofluorescence, primarily in the "V450" channel (i.e., excited by near-UV laser, 405 nm, and emitting in the 450 nm range). This is the detector you would use for Pacific Blue, ViViD, or Brilliant Violet 450 dyes. Interestingly, the problem only occurs in the tubes that have been sterilized by gamma-irradiation -- the unsterilized tubes are fine. The tubes are polypropylene, so it's quite possible that this issue may extend to other tubes that are similarly sterilized. The specific tubes come from USA Scientific. The "problem tubes" are catalog #1412-1410; the acceptable tubes are catalog #1412-0400. The sterilization process must be leading to the generation of a UV-excitable dye which leaches into the cells. The longer you incubate cells in the "problem" tubes, the brighter they become. Curiously, this doesn't always happen uniformly -- sometimes half the cells become bright and half do not. Perhaps it requires close proximity of the cell to the plastic. This heterogeneity is insidious -- it looks like you are generating new subsets of cells (rather than having a staining artefact).
- Stratedigm analyzers: how you can export an experiment or any part of it, including Instrument Settings, and then load it on any of the other Stratedigm machines. Please see the detailed instructions.