Welcome to the Yao Lab!
Probing Gene Transcription in a Cellular Context
We are generally interested in probing gene transcription and understanding its regulation in a cellular context. First, we are using state-of-the-art cell imaging approaches to visualize transcriptional output, kinetics and regulatory factors at single-copy promoter transgenes. Second, how gene transcription is under the regulation of spatial compartmentalization in the nucleus remains unclear. We are interested in studying the roles of nuclear lamina associations as a novel transcription repression mechanism.
Single cell analysis is uniquely suited for studying promoter regulation during development. The MyoD gene is a master regulatory gene of skeletal myogenesis in mammals, and elucidating its promoter regulation at the single cell level is an important step to fully understand the transcriptional mechanisms of muscle development.