Welcome to the Sweasy Lab

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Crystal structure of human DNA Polymerase Beta (pol β- 1bpy.pdb).

The ternary complex of Pol β is shown highlighting its four domains. The 8 kDa domain is shown in yellow; the thumb is in purple; the palm is in green; and the fingers domain in red.  Pol β prefers to bind to a one base pair gapped DNA substrate (colored by element).


 

The Sweasy Laboratory is part of the Radiobiology Section of the Department of Therapeutic Radiology. Our works focuses on the roles of DNA repair in the prevention of human disease and genomic instability. We purify and characterize DNA polymerases using presteady-state kinetics in order to understand how these enzymes chose the correct substrate for incorporation into DNA. Errors made by DNA polymerases result in mutations. 


Click on the images to see additional information about the structure of the Polymerase Beta (pol β- 1bpy.pdb).


alt textPOLB prostate cancer variant induces anchorage independent growth 

The Sweasy lab employs tissue culture systems to study the roles of genetic variants of DNA repair genes in genomic instability, cancer, and other diseases. A strong interest also concerns the impact of genetic variants of DNA repair genes on drug responses. We have also generated a number of genetically altered mice that have specific mutations in DNA repair genes, in order to understand how aberrant DNA repair contributes to cancer and other diseases.

Research

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Our research focuses on DNA repair, genomic instability and cancer; Meiosis; Inflammation and cancer; Single Nucleotide Polymorphisms (SNPs) in DNA repair genes and human disease; Fidelity of DNA synthesis.

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