Recent Publications


Re-visiting the trans insertion model for complexin clamping.

Krishnakumar SS, Li F, Coleman J, Schauder CM, Kümmel D, Pincet F, Rothman JE, Reinisch KM.

Elife. 2015 Apr 2;4 Epub 2015 Apr 2. PMID: 25831964


The principle of membrane fusion in the cell (Nobel lecture).

Rothman JE.

Angew Chem Int Ed Engl. 2014 Nov 17;53 (47) :12676-94. Epub 2014 Aug 1. PMID: 25087728


The Golgi ribbon structure facilitates anterograde transport of large cargoes.

Lavieu G, Dunlop MH, Lerich A, Zheng H, Bottanelli F, Rothman JE.

Mol Biol Cell. 2014 Oct 1;25 (19) :3028-36. Epub 2014 Aug 7. PMID: 25103235


Calcium sensitive ring-like oligomers formed by synaptotagmin.

Wang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV, Rothman JE.

Proc Natl Acad Sci U S A. 2014 Sep 23;111 (38) :13966-71. Epub 2014 Sep 8. PMID: 25201968


Common intermediates and kinetics, but different energetics, in the assembly of SNARE proteins.

Zorman S, Rebane AA, Ma L, Yang G, Molski MA, Coleman J, Pincet F, Rothman JE, Zhang Y.

Elife. 2014 Sep 1;3 :e03348. Epub 2014 Sep 1. PMID: 25180101


Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo.

Cho RW, Kümmel D, Li F, Baguley SW, Coleman J, Rothman JE, Littleton JT.

Proc Natl Acad Sci U S A. 2014 Jul 15;111 (28) :10317-22. Epub 2014 Jun 30. PMID: 24982161


A half-zippered SNARE complex represents a functional intermediate in membrane fusion.

Li F, Kümmel D, Coleman J, Reinisch KM, Rothman JE, Pincet F.

J Am Chem Soc. 2014 Mar 5;136 (9) :3456-64. Epub 2014 Feb 18. PMID: 24533674


Membrane adhesion dictates Golgi stacking and cisternal morphology.

Lee I, Tiwari N, Dunlop MH, Graham M, Liu X, Rothman JE.

Proc Natl Acad Sci U S A. 2014 Feb 4;111 (5) :1849-54. Epub 2014 Jan 21. PMID: 24449908


Conformational dynamics of calcium-triggered activation of fusion by synaptotagmin.

Krishnakumar SS, Kümmel D, Jones SJ, Radoff DT, Reinisch KM, Rothman JE.

Biophys J. 2013 Dec 3;105 (11) :2507-16. PMID: 24314081


Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes.

Pellett PA, Dietrich F, Bewersdorf J, Rothman JE, Lavieu G.

Elife. 2013 Oct 1;2 :e01296. Epub 2013 Oct 1. PMID: 24137546


COPI buds 60-nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function.

Thiam AR, Antonny B, Wang J, Delacotte J, Wilfling F, Walther TC, Beck R, Rothman JE, Pincet F.

Proc Natl Acad Sci U S A. 2013 Aug 13;110 (33) :13244-9. Epub 2013 Jul 30. PMID: 23901109


Stapled Golgi cisternae remain in place as cargo passes through the stack.

Lavieu G, Zheng H, Rothman JE.

Elife. 2013 Jun 4;2 :e00558. Epub 2013 Jun 4. PMID: 23755362


Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores.

Shi L, Howan K, Shen QT, Wang YJ, Rothman JE, Pincet F.

Nat Protoc. 2013 May;8 (5) :935-48. Epub 2013 Apr 18. PMID: 23598444


Single reconstituted neuronal SNARE complexes zipper in three distinct stages.

Gao Y, Zorman S, Gundersen G, Xi Z, Ma L, Sirinakis G, Rothman JE, Zhang Y.

Science. 2012 Sep 14;337 (6100) :1340-3. Epub 2012 Aug 16. PMID: 22903523


Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells.

Karatekin E, Rothman JE.

Nat Protoc. 2012 Apr 19;7 (5) :903-20. Epub 2012 Apr 19. PMID: 22517259


SNARE proteins: one to fuse and three to keep the nascent fusion pore open.

Shi L, Shen QT, Kiel A, Wang J, Wang HW, Melia TJ, Rothman JE, Pincet F.

Science. 2012 Mar 16;335 (6074) :1355-9. PMID: 22422984


Molecular mechanism of protein folding in the cell.

Rothman JE, Schekman R.

Cell. 2011 Sep 16;146 (6) :851-4. Epub 2011 Sep 9. PMID: 21907398


Complexin cross-links prefusion SNAREs into a zigzag array.

Kümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM.

Nat Struct Mol Biol. 2011 Jul 24;18 (8) :927-33. Epub 2011 Jul 24. PMID: 21785414


Complexin activates and clamps SNAREpins by a common mechanism involving an intermediate energetic state.

Li F, Pincet F, Perez E, Giraudo CG, Tareste D, Rothman JE.

Nat Struct Mol Biol. 2011 Jul 24;18 (8) :941-6. Epub 2011 Jul 24. PMID: 21785413


A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion.

Krishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE.

Nat Struct Mol Biol. 2011 Jul 24;18 (8) :934-40. Epub 2011 Jul 24. PMID: 21785412


The future of Golgi research.

Rothman JE.

Mol Biol Cell. 2010 Nov 15;21 (22) :3776-80. PMID: 21079007


Protein determinants of SNARE-mediated lipid mixing.

Ji H, Coleman J, Yang R, Melia TJ, Rothman JE, Tareste D.

Biophys J. 2010 Jul 21;99 (2) :553-60. PMID: 20643074


Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes.

Lavieu G, Orci L, Shi L, Geiling M, Ravazzola M, Wieland F, Cosson P, Rothman JE.

Proc Natl Acad Sci U S A. 2010 Apr 13;107 (15) :6876-81. Epub 2010 Mar 29. PMID: 20351264


A fast, single-vesicle fusion assay mimics physiological SNARE requirements.

Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE.

Proc Natl Acad Sci U S A. 2010 Feb 23;107 (8) :3517-21. Epub 2010 Feb 2. PMID: 20133592


Alternative zippering as an on-off switch for SNARE-mediated fusion.

Giraudo CG, Garcia-Diaz A, Eng WS, Chen Y, Hendrickson WA, Melia TJ, Rothman JE.

Science. 2009 Jan 23;323 (5913) :512-6. PMID: 19164750