Our main research focus includes:
- Coordination of Transcription and Splicing
- Cajal bodies and the macromolecular assembly of RNPs
- mRNP formation, composition and function
Cells express an astonishing variety of mRNA transcripts from a limited pool of genes. Across tissues and even among individuals, mRNAs produced from the same gene differ at their 5’ and 3’ ends as well as throughout the transcript body, enabling the expression of numerous protein products per gene. Transcript diversity is due to regulation of transcription and splicing, which we investigate in vivo.
We have established experimental systems in budding yeast, zebrafish embryos, and mammalian tissue culture cells to explore transcription and splicing regulation in a variety of biological contexts and with a diversity of tools, from imaging to genome-wide approaches. Our observations have provided novel insights into transcription and splicing mechanisms as well as principles of cellular organization that facilitate efficient gene expression.
EMBO Member Karla Neugebauer, Senior Group Leader at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, talks to Barbara Pauly about the differences in the research culture in Europe and North America, representation of women in academic scientific research, the Women in Life Science database, and the importance of soft skills for scientists.