2013

Schedule
Equipment
Software
Accessories

2013 Schedule of Events

Listed below within separate sections:

I ) Symposium
II) Equipment, software and technical demonstrations
III) Hands-on practicals

I) Symposium Schedule

Correlative Light Electron Microscopy - Part I TAC building auditorium N107 Tuesday, June 4th
2:30 – 3:30 PM	"Visualizing Cells and Viruses at Molecular Resolution: From Structure to Mechanism" Sriram Subramaniam, PhD Laboratory of Cell Biology National Cancer Institute, NIH
Coffee break
4:00 – 5:00 PM	"Correlative (i)PALM and EM Imaging" Gleb Shtengel, PhD Optical Physics and Instrument Design HHMI Janelia Farm, Asburn VA
Happy Hour to follow

Correlative Light Electron Microscopy - Part II TAC building auditorium N107 Wednesday June 5th
2:00 – 3:00 PM	"Correlative light and electron microscopy reveals new structure in the immunological synapse" Michael Dustin, PhD Department of Molecular Immunology Skirball Institute, NY
Coffee break
3:30 – 4:30 PM	"Development and Application of Labeling Technologies to Propel Correlated Multi-Scale Imaging of Cells and Tissues" Mark Ellisman, PhD National Center for Microscopy and Imaging Research University of California, San Diego
Happy Hour to follow

II) Equipment, Software and Technical Demonstrations and Lectures

Tuesday, June 4th
9:00 – 5:00 PM	Software Walk-in Clinics for post acquisition image analysis Imaris (TAC N213) Volocity (TAC N11) Amira and Avizo (TAC N209)
9:00 – 5:00 PM	Continued access to most instrumentation throughout the day TAC N221 - N239
9:00 – 9:45 AM	"Fundamentals of confocal microscopy" Technical lecture TAC N203 Joerg Bewersdorf, Yale University
9:45 – 10:30 AM	"Advances in Fluorescent Protein Technology" Technical lecture TAC N203 Michael Davidson, Florida State University
10:00–2:00 PM	Yale EM facility Open House SHM building IE-40
10:45-11:30 AM	Demo of SEM sample prep w Leica CPD300 Critical Point Dryer system Leica demo TAC N231
11:30–12:15 PM	Demo of JEOL Neoscope for benchtop SEM JEOL/Nikon demonstration TAC N221
12:15 – 1:00 PM	"Imaris XT & Imaris Open: Advanced image analysis enabling custom scripts within an expert forum" Imaris lecture TAC N213
1:00 – 2:00 PM	"Sample preparation for CLEM" Kim Rensing, PhD - Leica technical lecture TAC N231

Wednesday, June 5th
9:00 – 5:00 PM	Continued access to most instrumentation throughout the day TAC N221 - N239
10:00–10:45 AM	"Emerging camera technologies: a guide to CCDs and sCMOS cameras" Hamamatsu lecture TAC N203
10:00–11:00 AM	Demo of FEI/TILL Corrsight microscope for correlative light and EM imaging FEI/TILL photonics demonstration N237
11:00–12:00 AM	Leica EM HPM100 high pressure freezer and transfer demo Leica EM cryo sample preparation demo SHM building IE-40
12:00-1:00 PM	"Strategies for Correlative Microscopy in Biosciences Research" Kirk Czymmek, PhD - Zeiss technical lecture Tac Auditorium N107
12:30–1:30 PM	"Amira & Avizo software for visualization, analysis, & simulation with 3D light & EM data" FEI/VSG lecture TAC N209
1:00-2:00 PM	Demo of RMC computer controlled cryo ultramicrotome Greg Becker, PhD - RMC demonstration TAC N239
12:30 – 5:00 PM	Software Walk-in Clinics for post acquisition image analysis Imaris (TAC N213) Volocity (TAC N211) Amira and Avizo (TAC N209)
1:00 – 2:00 PM	"Basic fluorescence widefield deconstructed: understanding the building blocks of fluorescence microscopes" Nikon interactive session in TAC N221

Thursday, June 6th
9:00 - 5:00 PM	Continued access to most instrumentation throughout the day TAC N2221 - N239
10:00–11:00 AM	FEI Vitrobot demonstration FEI demonstration in TAC N237
10:00–11:00 AM	Demo of Lavision BioTec two-photon laser scanning microscope Staff demonstration in TAC S614
11:00–12:00 AM	Demo of Leica Ground State Depletion super resolution microscope Lecia demonstration in TAC N231
12:00-1:00 PM	Demo of iMIC multi-modal Andromeda/TIRF/FRAP system FEI/TILL Photonics demonstration in TAC N237
12:30–5:00 PM	Software Walk-in Clinics for post acquisition image analysis Imaris (TAC N213) Volocity (TAC N211) Amira and Avizo (TAC N209)
1:00 – 2:00 PM	Demo of Leica Ti:Sapphire STED microscope Lecia demonstration in SHM IE wing

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.

Tuesday, June 4th
10:30am–12:00	Small group class at confocals: "Optimizing confocal imaging" (for group #1) TAC N221 - N229
2:00 – 5:00 PM	Opportunity for individual practice - please reseve an instrument through the online scheduling system for guided use
Wednesday, June 5th
10:30am–12:00	Small group class at confocals: "Optimizing confocal imaging" (for group #2) TAC N221 - N229
2:00 – 5:00 pm	Opportunity for individual practice - please reseve an instrument through the online scheduling system for guided use
Thursday, June 6th
10:30am–12:00	Small group class at confocals: "Optimizing confocal imaging" (for group #3) TAC N221 - N229
2:00 – 5:00 PM	Opportunity for individual practice - please reseve an instrument through the online scheduling system for guided use

Equipment List

______________	Chameleon Vision II two-Photon laser with precompensation (680nm-1080nm)
______________	CorrSight microscope for efficient correlation of light and electron microscopy TF20 STEM/TEM w/ field gun, HAADF & EDEX detector, tomography enabled Can be reserved for up to 2 hrs for use in the Yale EM facility. T12 Technai Spirit TEM for analysis of both inorganic and organic samples Can be reserved for up to 2 hrs for use in the Yale EM facility. iMIC multi-modal microscope w/ spinning disk confocal & TIRF/FRAP capability Can be reserved for up to 2 hrs FEI Vitrobot vitrification robot for single particle analysis or cryo-tomography
______________	JEOL Neoscope bench top scanning electron microscope Can be reserved for 1 hr
______________	Two-photon laser scanning microscope with NDD PMTs outfitted with Chameleon Vision II laser (see above; can be reserved for 1 hr)
______________	Leica TCS Ti:Sapphire STED for super resolution microscopy Can be reserved for 2 hrs. Note! Sample prep is critical! See this guide for advice Leica GSD ground state depletion super resolution TIRF microscope Can be reserved for 2 hrs. Note! Sample prep is critical! See this guide for advice Leica EM HPM100 high pressure freezer & EM AFS2 freeze substitution unit The HPM100 can be reserved for 1.5 hour for use in Yale EM facility, although the time required for freeze substitution is prohibitive during the workshop. Leica Automated Critical Point Dryer EM CPD300 Can be reserved for 1.5 hrs. Note! Samples must be fixed prior to use of this unit & subsequent coating. See this guide for suggested sample prep Leica Automatic Plunge Freezing EM GP Leica Low Vacuum Coater EM ACE200
______________	A1Rsi confocal w spectral detectors & resonant scanners for ROI studies during imaging Can be reserved for 1 hr Eclipse Ni widefield for fluorescence
______________	Olympus FV1200 confocal Can be reserved for 1 hr
______________	RMC PT-PCZ/CRX computer controlled cryo-ultramicrotome
	ZEISS LSM710 laser scanning confocal w/ superb image quality & CLEM capabilities Can be reserved for 1 hr Axio Zoom.V16 to image large samples with amazing resolution & brilliance

Featured Software for Post Acquisition Image Analysis

	Volocity Visualization and Quantitation software View your cells in 3D and 4D with Volocity Visualization. Find, measure, track and chart objects in your samples.
	Imaris Track and Measurement Pro software Enables 3D and 4D visualization, quantification, tracking, and tracing of multi-channel and time series data.
	Amira 3D analysis software for Life Sciences Multifaceted 3D software platform for visualizing, manipulating and understanding biomedical data coming from all types of sources and modalities. Avizo 3D analysis software for scientific and industrial data Avizo is packaged in different editions with optional eXtension modules for tailored user interface and specific features for a variety of applications

Featured Accessories

	Molecular Probes / Invitrogen FluoCells prepared slide #1 bovine pulmonary artery endothelial cells. Brillant long-lasting mounted cells fluorescently stained for mitochondria (MitoTracker Red CMXRos), F-actin (BODIPY FL phallacidin), and nuclei (DAPI) FluoCells prepared slide #3 16 um thick mouse kidney. Prepared slides fluorescently stained for wheatgerm agglutinin (AlexaFluor 488), phalloidin (AlexaFluor 568), and nuclei (DAPI). NucRed live 647 ReadyProbes nuclear stain kit. For the rapid nuclear staining of live and fixed cells with a far-red emission in a liquid ready-to-use formulation in convenient dropper bottles BODIPY FL C5-Ganglioside GM1 complexed to BSA. A fluorescent reagent that aids in the identification of lipid rafts. The BSA-complexed lipophilic probe can be directly dissolved in water. BODIPY TR Ceramide complexed to BSA. This red fluorescent probe can be used to examine sphingolipid transport and metabolism.
	Electron Microscopy Sciences 50% glutaraldehyde 10ml x 10/box 16% paraformaldehyde 10ml x 10/box EM grids: FCF100-Cu and FCF100-Ni Embedding medium: EMBed-812 kit
	Hamamatsu PMTs, CCDs, and sCMOS Cameras
	Warner Instruments WillCo glass bottom culture dishes. High quality glass bottom polystyrene dishes pre-sterilized in both 35 mm and 50 mm diameters.