2013

2013 Schedule of Events

Listed below within separate sections:

I ) Symposium
II) Equipment, software and technical demonstrations
III) Hands-on practicals

I) Symposium Schedule

Correlative Light Electron Microscopy - Part I
TAC building auditorium N107
Tuesday, June 4th

2:30 – 3:30 PM"Visualizing Cells and Viruses at Molecular Resolution: From Structure to Mechanism"
Sriram Subramaniam, PhD
Laboratory of Cell Biology
National Cancer Institute, NIH
Coffee break
4:00 – 5:00 PM"Correlative (i)PALM and EM Imaging"   
Gleb Shtengel, PhD
Optical Physics and Instrument Design
HHMI Janelia Farm, Ashburn VA
Happy Hour to follow

Correlative Light Electron Microscopy - Part II

TAC building auditorium N107 
Wednesday June 5th 
2:00 – 3:00 PM  "Correlative light and electron microscopy reveals new structure in the immunological synapse"
Michael Dustin, PhD
Department of Molecular Immunology 
Skirball Institute, NY
Coffee break
3:30 – 4:30 PM"Development and Application of Labeling Technologies to Propel Correlated Multi-Scale Imaging of Cells and Tissues"

Mark Ellisman, PhD
National Center for Microscopy and Imaging Research
University of California, San Diego

Happy Hour to follow


II) Equipment, Software and Technical Demonstrations and Lectures


Tuesday, June 4th
9:00 – 5:00 PMSoftware Walk-in Clinics for post acquisition image analysis

Imaris (TAC N213)
Volocity (TAC N11)

Amira and Avizo (TAC N209) 

9:00 – 5:00 PM 

Continued access to most instrumentation throughout the day

TAC N221 - N239

9:00 – 9:45 AM"Fundamentals of confocal microscopy" 
Technical lecture TAC N203
Joerg Bewersdorf, Yale University
9:45 – 10:30 AM"Advances in Fluorescent Protein Technology" 
Technical lecture TAC N203
John Allen, Florida State University
10:00–2:00 PM Yale EM facility Open House

SHM building IE-26

10:45-11:30 AM

Demo of SEM sample prep w Leica CPD300 Critical Point Dryer system

Leica demo TAC N231

11:30–12:15 PM

Demo of JEOL Neoscope for benchtop SEM

JEOL/Nikon demonstration TAC N221

12:15 – 1:00 PM"Imaris XT & Imaris Open: Advanced image analysis enabling custom scripts within an expert forum"

Imaris lecture TAC N213

1:00 – 2:00 PM"Sample preparation for CLEM"
Kim Rensing, PhD - Leica technical lecture TAC N203
Wednesday, June 5th
9:00 – 5:00 PMContinued access to most instrumentation throughout the day

TAC N221 - N239

10:00–10:45 AM

"Emerging camera technologies: a guide to CCDs and sCMOS cameras"

Megan MacNeil, Hamamatsu technical lecture TAC N203

10:00–11:00 AM

Demo of FEI/TILL Corrsight microscope for correlative light and EM imaging

FEI/TILL photonics demonstration N237
11:00–12:00 AM

Leica EM HPM100 high pressure freezer and transfer demo 

Leica EM cryo sample preparation demo SHM building IE-40

12:00-1:00 PM

"Strategies for Correlative Microscopy in Biosciences Research"

Kirk Czymmek, PhD - Zeiss technical lecture Tac Auditorium N107

12:30–1:30 PM 

"Amira  & Avizo software for visualization, analysis, & simulation with 3D light & EM data"

FEI/VSG lecture TAC N209

1:00-2:00 PM

Demo of RMC computer controlled cryo ultramicrotome

Greg Becker, PhD - RMC demonstration TAC N239

12:30 – 5:00 PM

Software Walk-in Clinics for post acquisition image analysis
Imaris (TAC N213)
Volocity (TAC N211)

Amira and Avizo (TAC N209)

1:00 – 2:00 PM  "Basic fluorescence widefield deconstructed: understanding the building blocks of fluorescence microscopes" 
Nikon interactive session in TAC N221
Thursday, June 6th
9:00 - 5:00 PM  Continued access to most instrumentation throughout the day
TAC N2221 - N239  
10:00–11:00 AM

FEI Vitrobot vitrification robot demonstration

FEI demonstration in TAC N237

10:00–11:00 AM

Demo of Lavision BioTec two-photon laser scanning microscope

Staff demonstration in TAC S614

11:00–12:00 AMDemo of Leica Ground State Depletion super resolution microscope 
Lecia demonstration in TAC N231
12:00-1:00 PM

Demo of iMIC multi-modal Andromeda/TIRF/FRAP system

FEI/TILL Photonics demonstration in TAC N237

12:30–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis

Imaris (TAC N213) 
Volocity (TAC N211)

Amira and Avizo (TAC N209)

1:00 – 2:00 PMDemo of Leica Ti:Sapphire STED microscope 

Lecia demonstration in SHM IE wing

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.

Tuesday, June 4th
10:30am–12:00Small group class at confocals: "Optimizing confocal imaging" 
(for group #1)
TAC N221 - N229
2:00 – 5:00 PMOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use

Wednesday, June 5th

10:30am–12:00   Small group class at confocals: "Optimizing confocal imaging" 
(for group #2)
TAC N221 - N229
2:00 – 5:00 pmOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use
Thursday, June 6th
10:30am–12:00Small group class at confocals: "Optimizing confocal imaging" 
(for group #3)
TAC N221 - N229
2:00 – 5:00 PMOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use

Equipment List

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  • Olympus FV1200 confocal
    Can be reserved for 1 hr

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  • ZEISS LSM710 laser scanning confocal w/ superb image quality & CLEM capabilities
    Can be reserved for 1 hr
  • Axio Zoom.V16 to image large samples with amazing resolution & brilliance
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Featured Software for Post Acquisition Image Analysis


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  • Gatan 3view software
    3D rendering and quantiative measurement software suitable for analysis of serial block-face EM and correlative microscopy

Featured Accessories

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Molecular Probes / Invitrogen



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Warner Instruments