2016 Schedule of Events

Listed below within separate sections:

I ) Symposium 
II) Equipment, software and technical demonstrations
III) Hands-on practicals 

I) Symposium Schedule

Novel Fluorescent Probes and Labeling Techniques - Part I
TAC building auditorium N107
Tuesday afternoon, June 14th
2:00–3:00 PM"Spontaneously blinking fluorophore based intramolecular spirocyclization for live-cell super-resolution imaging"
Yasuteru Urano, PhD
Dept of Chemical Biology and Molecular Imaging
University of Tokyo
3:00–3:30 PM"Live-cell nanoscopy of the golgi and the ER with bioorthogonal ceramide probes"
Roman Erdmann, PhD
Depts of Chemisty and Cell Biology
Yale University
Coffee break
4:00–5:00 PM"Designing brighter dyes for advanced fluorescence microscopy
Luke Lavis, PhD 
Section of Chemical and Molecular Tool Development
HHMI Janelia Farm, Ashburn VA
Happy Hour to follow
  
Novel Fluorescent Probes and Labeling Techniques - Part II
TAC building auditorium N107
Wednesday morning, June 15th
9:00–9:30 AM"Engineering better optical neuronal activity indicators"
Vincent Pieribone, PhD
Depts of Cellular and Molecular Physiology and Neuroscience
John B. Pierce Laboratory at Yale University
9:30-10:30 AM

"Single molecule imaging of genome structure and gene expression"
Alistair Boettiger, PhD
Dept of Chemistry and Chemical Biology
Harvard University

Coffee break 
11:00–12:00 PM"Advances in molecular probe-based labeling tools and their application to correlated light, electron and X-ray microscopies"
Daniela Boassa, PhD
National Center for Microscopy and Imaging Research
University of California, San Diego


 

II) Equipment, Software and Technical Demonstrations and Lectures

Tuesday, June 14th
9:00–5:00 PMSoftware Walk-in Clinic for post acquisition image analysis (except 11:30 am-12:30)
Imaris (TAC N213)
10:00–5:00 PMContinued access to most participating instruments throughout the day
See the Equipment page for locations on campus and the FAQs page for information on reservations
9:15–10:00 AM"Fundamentals of optical configurations for spectral fluorescence detection"
Technical lecture TAC N203
Joerg Bewersdorf, Dept Cell Biology, Yale University
10:0–10:45 AM

"Fundamentals of fluorescent protein and dye considerations"

Technical lecture TAC N203

Derek Toomre, Dept Cell Biology, Yale University

11:00 AM–12:00 PMTEM demonstration within Yale's EM facility
Xinran Liu, facility Director - SHM IE-26 
11:30 AM–12:30
"3D/4D image visualization and analysis with Imaris 8" 
Brian Thompson, Imaris technical lecture - TAC N221
12:30-1:00 PM

"One probe, two answers: Simultaneous light, superresolution, and electron microscopic immunolabeling with FluoroNanogold"
Richard Powell, NanoProbes - TAC N221

1:00–2:00 PMDemo of Leica SP8 STED super resolution
Leica demonstration in SHM IE wing 
2:00-3:00 PMDemo of Lavision BioTec two-photon laser scanning microscope
Staff demonstration in TAC S614
  
Wednesday, June 15th
10:00–5:00 PMContinued access to most particpating instruments throughout the day
See the Equipment page for locations on campus and the FAQs page for information on reservations
1:00–2:00 PM"Sample preparation for Correlative light and EM imaging"
Kirk Czymmek, technical lecture in TAC N107
1:00–2:00 PMDemo of Lavision BioTec two-photon laser scanning microscope
Staff demonstration in TAC S614
2:00–3:00 PMYale EM and Cryo-EM facility Tour
Xinran Liu, facility Director - SHM IE-26
2:00-2:30 PM

"Orthogonal covalent live cell labeling using HaloTagTM Technology"
Mark McDougall, Promega technical lecture in TAC N203

2:30-3:00 PM"Using branch DNA technology to take single-cell RNA analysis to the next level in microscopy and flow cytometry"
 Noah Alberts-Grill, Affymetrix technical lecture- TAC N203
3:00–4:00 PMDemo of Leica SP8 STED super resolution   
Leica demonstration in SHM IE wing
3:00-3:30 PM"Illuminating cell structure and organelle dynamics"
Chris Langsdorf, Molecular Probes/ThermoFisher presentation - TAC N203
3:30-4:00 PM"Engineering fluorescence to visualize cell health, growth and function"
Chris Langsdorf, Molecular Probes/ThermoFisher presentation - TAC N203
4:00-4:30 PM"Quantative imaging flow cytometry applications"   
Darin Fogg, Amnis technical lecture - TAC N203
4:30-5:00 PMDemo of Amnis Imagestream Mark II imaging flow cytometer
Staff demonstration in TAC S613

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.
Tuesday, June 14th
10:15 AM - 11:15PMSmall group practical session on spectral imaging and spectra unmixing
(for group #1) at Leica confocals with spectral detectors in SHM IE wing  
11:15 AM - 12:15PMSmall group practical session on spectral imaging and spectra unmixing
(for group #2) at Leica confocals with spectral detectors in SHM IE wing 
2:00–5:00 PMOpportunity for individual practice - please reserve an instrument through the online scheduling system for guided use
  
Wednesday, June 15th
10:15 AM - 11:15PMSmall group practical session on spectral imaging and spectra unmixing
(for group #3) at Leica confocals with spectral detectors in SHM IE wing 
11:15 AM - 12:15PMSmall group practical session on spectral imaging and spectra unmixing
(for group #4) at Leica confocals with spectral detectors in SHM IE wing
2:00–5:00 PMOpportunity for individual practice - please reserve an instrument through the online scheduling system for guided use