2015

2015 Schedule of Events

Although the symposia speakers are confirmed, please note that some technical lectures and demonstrations are tentative. Additional events are likely to be added.

Listed below within separate sections:

I ) Symposium
II) Equipment, software and technical demonstrations
III) Hands-on practicals

I) Symposium Schedule

Visualizing Sub-Cellular Structures in 3D electron tomography and cryo-EM  
TAC building auditorium N107
Tuesday, June 2nd 

2:00 – 3:00 PM"Cryo-EM studies on Microtubule-MAP interactions in vitro and in situ"  
Andreas Hoenger, PhD
Dept of Molecular Cellular and Developmental Biology 
University of Colorado at Bolder 

3:00 – 3:30 PM

"TBD"
Chuck Sindelar, PhD 
Dept of Molecular Biophysics and Biochemistry  
Yale University

Coffee break
4:00 – 5:00 PM"TBD"   
Wah Chiu, PhD
Dept of Biochemistry and Molecular Biology 
Baylor College of Medicine, Houston TX 
Happy Hour to follow

Light Sheet Microscopy  

TAC building auditorium N107 
Wednesday June 3rd  
2:00 – 3:00 PM  "TBD"
Hari Schroff, PhD
Section of High Resolution Imaging  
NIH/NIBIB 
Coffee break

3:00 – 3:30 PM

"TBD
Daniel Colon-Ramos, PhD
Dept of Cell Biology
Yale University
4:00 – 5:00 PM"TBD"
Wesley Legant, PhD
Optical Physics
HHMI Janelia Farm, Ashburn VA 

Happy Hour to follow


II) Equipment, Software and Technical Demonstrations and Lectures


Tuesday, June 2nd
9:00 – 5:00 PMSoftware Walk-in Clinics for post acquisition image analysis

Imaris (TAC N213)
Volocity (TAC N11)
Amira and Avizo (TAC N209)

9:00 – 5:00 PM 

Continued access to most instrumentation throughout the day
TAC N221 - N239
9:15 –10:00 AM"Fundamentals of confocal microscopy" 
Technical lecture TAC N203
Joerg Bewersdorf, Yale University
10:45-11:30 AM

Demo of SEM sample prep w Leica CPD300 Critical Point Dryer system
Leica demo TAC N231

11:00–12:00 PM

Yale EM facility Tour
SHM building IE-26 

11:30–12:15 PM

Demo of JEOL Neoscope for benchtop SEM

JEOL/Nikon demonstration TAC N221

12:15 – 1:00 PM"Imaris XT & Imaris Open: Advanced image analysis enabling custom scripts within an expert forum"

Imaris lecture TAC N213

1:00 – 2:00 PM"TBD"
Wednesday, June 3rd
9:00 – 5:00 PMContinued access to most instrumentation throughout the day

TAC N221 - N239

9:15–9:45 AM

"Light Sheet/SPIM microscope design considerations"

Thomas Pingle, LaVision BioTec technical lecture TAC N203

9:45–10:15 AM

"Sample Preparation of Thick Tissues for Light Sheet imaging"

TBD, ___ technical lecture TAC N203


11:00–12:00 AM

Leica EM HPM100 high pressure freezer and transfer demo 

EM cryo sample preparation demo SHM building IE-40

12:00-1:00 PM

TBD 


12:30–1:30 PM 

"Amira  & Avizo software for visualization, analysis, & simulation with 3D light & EM data"

FEI/VSG lecture TAC N209

1:00-2:00 PM

TBD 


12:30 – 5:00 PM

Software Walk-in Clinics for post acquisition image analysis
Imaris (TAC N213)
Volocity (TAC N211)

Amira and Avizo (TAC N209)

1:00 – 2:00 PM  "Basic fluorescence widefield deconstructed: understanding the building blocks of fluorescence microscopes" 
Nikon interactive session in TAC N221
Thursday, June 4th
9:00 - 5:00 PM  Continued access to most instrumentation throughout the day
TAC N2221 - N239  
10:00–11:00 AM

FEI Vitrobot vitrification robot demonstration

FEI demonstration in TAC N237

10:00–11:00 AM

Demo of Lavision BioTec two-photon laser scanning microscope

Staff demonstration in TAC S614

11:00–12:00 AM

Yale EM facility Tour 


SHM building IE-26 

12:30–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis

Imaris (TAC N213) 
Volocity (TAC N211)

Amira and Avizo (TAC N209)

1:00 – 2:00 PMDemo of Leica Ti:Sapphire STED microscope 

Lecia demonstration in SHM IE wing

1:00 – 2:00 PM

"Collaborative Opportunities with the Leica Center of Excellence at Yale

Doug Giszczynski, Leica in TAC N203

3:00 – 5:00 PMTour of Leica Center of Excellence on Yale's West Campus (shuttle required) 

Validimir ___, 

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.

Tuesday, June 2nd
10:30am–12:00Small group class at confocals: "Optimizing confocal imaging"  
(for group #1)
TAC N221 - N229
2:00 – 5:00 PMOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use

Wednesday, June 3rd

10:30am–12:00   Small group hands-on class at Light Sheet microscopes  
(for group #2)
TAC N221 - N229
2:00 – 5:00 pmOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use
Thursday, June 4th
10:30am–12:00Small group hands-on class at Light Sheet microscopes 
(for group #3)
TAC N221 - N229
10:30am–12:00

Small group class at confocals: "Optimizing confocal imaging"

(for group #3)  


TAC N221 - N229

2:00 – 5:00 PMOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use

Equipment List

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  • Gatan high sensitivity camera for EM
    available for demonstration in Yale EM facility

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  • Olympus FV1200 confocal
    Can be reserved for 1 hr

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  • ZEISS LSM710 laser scanning confocal w/ superb image quality & CLEM capabilities
    Can be reserved for 1 hr
  • Axio Zoom.V16 to image large samples with amazing resolution & brilliance
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Featured Software for Post Acquisition Image Analysis


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  • Gatan 3view software
    3D rendering and quantiative measurement software suitable for analysis of serial block-face EM and correlative microscopy

Featured Accessories

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Molecular Probes / Invitrogen



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Warner Instruments