2015

2015 Schedule of Events

Although the symposia speakers are confirmed, please note that some technical lectures and demonstrations are tentative. Additional events are likely to be added.

Listed below within separate sections:

I ) Symposium
II) Equipment, software and technical demonstrations
III) Hands-on practicals

 

I) Symposium Schedule

Visualizing Sub-Cellular Structures in 3D electron tomography and cryo-EM
TAC building auditorium N107
Tuesday, June 2nd
2:30–3:30 PM"Cryo-EM for molecular and cellular biology research" 
Wah Chiu, PhD 
National Center for Macromolecular Imaging
Baylor College of Medicine, Houston TX
3:30–4:00 PM"Sharpening our view of molecular machines that attach to filaments"
Chuck Sindelar, PhD
Dept of Molecular Biophysics and Biochemistry
Yale University
Coffee break 
4:30–5:30 PM"Cryo-EM studies of microtubule-MAP interactions in vitro and in situ"
Andreas Hoenger, PhD
Dept of Molecular, Cellular and Developmental Biology
University of Colorado at Bolder
Happy Hour to follow
  
Light Sheet Microscopy
TAC building auditorium N107
Wednesday June 3rd
2:00–3:00 PM"Sharper, faster, deeper, gentler: 4D imaging technologies for interrogating live cells and embryos"
Hari Shroff, PhD
Section of High Resolution Imaging
NIH/NIBIB
3:00–3:30 PM"Towards the living connectome: Systematic single cell analysis of neurodevelopment"
Daniel Colon-Ramos, PhD
Dept of Cell Biology
Yale University
Coffee break 
4:00–5:00 PM"Lattice light sheet microscopy: imaging molecules, cells, and embryos at high spatiotemporal resolution"
Wesley Legant, PhD
Optical Physics
HHMI Janelia Farm, Ashburn VA
Happy Hour to follow

II) Equipment, Software and Technical Demonstrations and Lectures

Tuesday, June 2nd
9:00–5:00 PMSoftware Walk-in Clinic for post acquisition image analysis
Imaris (TAC N213)
9:00–5:00 PMContinued access to most instrumentation throughout the day
TAC N221 - N239
9:15–10:00 AM"Fundamentals of confocal microscopy"
Technical lecture TAC N203
Joerg Bewersdorf, Yale University
10:45–11:30 AMDemo of SEM sample prep w Leica CPD300 Critical Point Dryer system
Leica demo - TAC N221 
11:00 AM–12:00 PMYale EM and Cryo-EM facility Tour
Xinran Liu, facility Director - SHM IE-40 
11:30 AM–12:15 PMDemo of JEOL Neoscope for benchtop SEM
JEOL/Nikon demonstration - TAC N223 
12:15–1:00 PM"3D/4D image visualization and analysis with Imaris 8" 
Matthew Gastinger, Imaris technical lecture - TAC N213
1:00-2:00 PM

"Correlating light and EM of cryofixed samples using Leica EM Cryo-CLEM"
Levi Felts, Leica EM division, technical lecture in TAC N221

1:00–2:00 PM"In vivo and cleared tissue imaging with the Zeiss Lightsheet Z.1"
Scott Olenych, Zeiss, technical lecture in TAC N231 
  
Wednesday, June 3rd
9:00–5:00 PMContinued access to most instrumentation throughout the day
TAC N221 - N239
9:15–9:45 AM"Light Sheet/SPIM microscope design considerations"
Thomas Pingel, LaVision BioTec technical lecture TAC N229 
9:45–10:15 AM"Sample Preparation of Thick Tissues for Light Sheet imaging"
Uwe Schroer, LaVision BioTec technical lecture TAC N229 
11:00–12:00 AMLeica EM HPM100 high pressure freezer and transfer demo
Leica demo of cryo sample prep in Yale EM facility - SHM IE-40
1:00–2:00 PM"Basic fluorescence widefield deconstructed: understanding the building blocks of fluorescence microscopes"

Nikon interactive session with Stan Thorburn in TAC N223

1:00-2:00 PM"Leica Digital Lightsheet Imaging - the Vertical Turn"   
Florian Fahrbach, Leica Applications Development, Mannheim Germany - TAC N231
  
Thursday, June 4th
9:00–5:00 PMContinued access to most instrumentation throughout the day
TAC N2221 - N239
10:00–11:00 AMFEI Vitrobot vitrification robot demonstration
Marc Llaguno, Yale EM facility - SHM IE-26 
10:00–11:00 AMDemo of Lavision BioTec two-photon laser scanning microscope
Staff demonstration in TAC S614
11:00 AM-12:00 PM    

Special Announcement and technical lecture 
"Super resolution sample preparation for Yale's new Leica STED 3x microscope"
Louise Bertrand, Leica specialist - TAC N231

11:00 AM–12:00 PMYale EM and Cryo-EM facility Tour
Xinran Liu, facility Director - SHM IE-40 
1:00–2:00 PMDemo of Leica Ti:Sapphire STED microscope
Lecia demonstration in SHM IE wing
1:00–1:30 PM

"Collaborative Opportunities with the Leica Center of Excellence at Yale West Campus"
Doug Giszczynski, Leica Regional Manager - TAC N231  
Vladimir Polejaev, West Campus Imaging Core Director

2:00–2:30 PM"High Throughput Fluorescence Microscopy at the Yale Center for Molecular Discovery: Rapid Methods for Optimizing Your Immunostaining Protocol and Quantifying Images in 96- and 384-well Plates"
Yulia Surovtseva and Janie Merkel Yale Center for Molecular Discovery - TAC N211 

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.
Tuesday, June 2nd
10:30 AM - 12:00PMSmall group hands-on class at Light Sheet microscopes
(for group #1) - THIS CLASS IS NOW FULL AND THE WAITING LIST VERY LENGTHY.  
LaVision BioTec within TAC N223 or West Campus Leica facility via dedicated shuttle bus
2:00–5:00 PMOpportunity for individual practice - please reserve an instrument through the online scheduling system for guided use
  
Wednesday, June 3rd
10:30 AM–12:00 PMSmall group hands-on class at Light Sheet microscopes
(for group #2) - THIS CLASS IS NOW FULL, BUT ADDITIONAL NAMES FOR THE WAIT LIST ARE STILL BEING ACCEPTED.
LaVision BioTec within TAC N223 or West Campus Leica facility via dedicated shuttle bus 
2:00–5:00 PMOpportunity for individual practice - please reserve an instrument through the online scheduling system for guided use
  
Thursday, June 4th
10:30 AM–12:00 PMSmall group hands-on class at Light Sheet microscopes
(for group #3) - THIS CLASS IS NOW FULL AND THE WAITING LIST VERY LENGTHY.
LaVision BioTec within TAC N223 or West Campus Leica facility via dedicated shuttle bus 
2:00–5:00 PMOpportunity for individual practice - please reserve an instrument through the online scheduling system for guided use

Equipment List

icon-Coherent.png

______________

______________

  • Tecnai 200kV STEM/TEM w/ field emission gun, HAADF detector, hardware/software for dual-tilt tomography
    Can be reserved for up to 2 hrs for use in the Yale EM facility.
  • Tecnai T12 TEM (120 kV) in conjunction with Leica Cryo-CLEM system
    Can be reserved for up to 2 hrs for use in the Yale EM facility.
  • FEI Vitrobot vitrification robot to prepare cell or protein samples
    For subsequent cryo-TEM and single particle analysis or cryo electron tomography



  • Gatan high sensitivity camera for EM
    available for demonstration in Yale EM facility

______________

LaVision-for-web.png

______________

Leica-for-web.png

______________

Nikon-for-web.png

______________

Zeiss_web.png
  • The Zeiss Light sheet microscope will not be installed on the Yale campus, but can be viewed at the Zeiss center in Thornwood, NY which is 1.5 hours away by car, or via Zeiss provided bus.
    Individuals interested in seeing a demonstration of this instrument at the Thornwood center should contact Geoff Guimaraes directly via email (geoff.guimaraes@zeiss.com).
S

Featured Software for Post Acquisition Image Analysis


BPLogo-for-web.png
  • Gatan 3view software
    3D rendering and quantiative measurement software suitable for analysis of serial block-face EM and correlative microscopy

Featured Accessories

Molecular probes.jpg

Molecular Probes / Invitrogenempty
emptyemptyemptyemptyemptyemptyemptyempty


empty

SunJin web.gif

SunJin Lab

Vendor suggested protocols for immunostaining can be downloaded here using 1) a SOP for RapiClear, 2) passive CLARITY for confocal, or 3) passive CLARITY for Lightsheet imaging. Tissues with endogenous fluorescent proteins can instead directly follow the shorter protocols listed in the product information files below.