2017 Schedule of Events

June 6-7th 2017

The tentative schedule is listed below within separate sections:

I ) Symposium 
II) Equipment, software and technical demonstrations
III) Hands-on practicals 

I) Symposium Schedule

Image Analysis
TAC building auditorium N107
Tuesday afternoon, June 6th
1:00–2:00 PM"TBD"
Anne Carpenter, PhD
Imaging Platform Center
Broad Institute of Harvard and MIT
2:00–3:00 PM"TBD"
Gaudenz Danuser, PhD
Dept of Bioinformatics
University of Texas Southwestern Medical Center
Coffee break
3:30-4:00"TBD"  
Fred Sigworth, PhD  
Dept of Molecular and Cellular Physiology 
Yale University
4:00–5:00 PM"TBD
Eija Jokitalo, PhD 
Institute of Biotechnology
University of Helsinki, Finland
Happy Hour to follow
  
High-dimensional imaging
TAC building auditorium N107
Wednesday afternoon, June 7th
1:00-2:00 PM"miCAT image cytometry analysis toolbox for the systematic analysis of cellular networks in highly multiplexed tissue images."  
Denis Schapiro, PhD  
Institute of Molecular Life Sciences 
University of Zurich, Switzerland 
2:00–3:00 PM"Quantitative cell subsetting by morphology using Amnis imaging flow cytometry"
Kathleen McGrath, PhD
Center for Pediatric Biomedical Disease
University of Rochester, NY
Coffee break

 

3:30-4:30 PM"Highly multiplexed fluorescent imaging by simplified antibody stripping for 18+ channel staining" 
Giorgio Cattoretti, MD 
Dept of Medicine 
Universata degli Studi di Milano-Biococca

II) Equipment, Software and Technical Demonstrations and Lectures

Tuesday, June 6th
9:00–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis (except during relevant lectures)
Imaris (TAC N209), Amira (TAC N211), InCell (TAC N229) 
9:00–5:00 PMContinued access to most participating instruments throughout the day
See the Equipment page for locations on campus and the FAQs page for information on reservations
9:30–10:30 AM"Correlative light and Cryo electron microscopy"
Louise Bertrand, Leica lecture and demo of cryo CLEM - TAC N223 
10:30–11:00 AMDemo of Nikon Eclipse Ti2 features for high-throughput and large field of view 
Nikon demonstration in TAC N227
10:30–11:00 AMDemonstration of FEI Vitrobot for cryo sample preparation 
Staff demonstration within Yale EM facility - SHM IE-26
11:00 AM–12:00 PMDemonstration of cryo-prepared sample TEM using FEI ___ 
Staff demonstration within Yale EM facility - SHM IE-26
11:15 AM–12:00 PM"GE InCell high-throughput imaging and image analysis"  
Damien McCann, GE technical lecture and demonstration - TAC N229
11:30 AM-12:00 PM"Amira image analysis"  
FEI technical lecture - TAC N211
12:00–12:45 PM"LSM 880-Airyscan - improving resolution and signal-to-noise in cryo-fluorescence microscopy as part of a correlative EM-LM workflow"   
Zeiss technical lecture and demonstration - TAC N229
12:00–12:30 PM
"3D/4D image visualization and analysis with Imaris 8" 
Brian Thompson, Imaris technical lecture - TAC N221
  
Wednesday, June 7th
9:00–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis (except during relevant lectures) 
Imaris (TAC N209), Amira (TAC N211), InCell (TAC N229)
9:00–5:00 PMContinued access to most participating instruments throughout the day
See the Equipment page for locations on campus and the FAQs page for information on reservations
9:30–10:30 AM"Cryo EM tomography"
____, FEI technical lecture in TAC N203 
9:30–10:30 AMDemonstration of Leica Cryo-CLEM microscope and accessories 
Leica demo - TAC N223
10:30-11:00 AM"Super-resolution imaging with Leica STED3X"    
Sarah Crowe, Leica - TAC N203
11:00 AM-12:00 PMDemonstration of cryo-prepared sample TEM using FEI ___  
Staff demonstration within Yale EM facility - SHM IE-26
11:00–11:45 AMDemonstration of Lavision BioTec two-photon laser scanning microscope
Staff demonstration in TAC S614
11:30 AM–12:30 PM"Abberior STEDYCON conversion of epifluorescence microscopes to a STED system" 
Gerald Donnert, Abberior - TAC N227

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.
Tuesday, June 6th
9:30 AM - 10:30AMSmall group practical session on high-throughput imaging
(for group #1) at participating HT widefields in the North TAC wing  
10:30 AM - 11:30AMSmall group practical session on imaging flow cytometry
(for group #2) at the Amnis Imagestream MkII in TAC S613 
1:00–5:00 PMOpportunity for individual practice - please reserve an instrument through the online scheduling system for guided use
  
Wednesday, June 7th
9:30 AM - 10:30AMSmall group practical session on high-throughput imaging
(for group #3) at participating HT widefields in the North TAC wing
10:30 AM - 11:30AMSmall group practical session on imaging flow cytometry
(for group #4) at the Amnis Imagestream MkII in TAC S613 
1:00–5:00 PMOpportunity for individual practice - please reserve an instrument through the online scheduling system for guided use