2015

2015 Schedule of Events

Although the symposia speakers are confirmed, please note that some technical lectures and demonstrations are tentative. Additional events are likely to be added.

Listed below within separate sections:

I ) Symposium
II) Equipment, software and technical demonstrations
III) Hands-on practicals

I) Symposium Schedule

Visualizing Sub-Cellular Structures in 3D electron tomography and cryo-EM
TAC building auditorium N107
Tuesday, June 2nd
9:00–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis
Imaris (TAC N213)
Volocity (TAC N11)
Amira and Avizo (TAC N209)
3:00–3:30 PM"Sharpening our view of molecular machines that attach to filaments"
Chuck Sindelar, PhD
Dept of Molecular Biophysics and Biochemistry
Yale University
Coffee break
4:00–5:00 PM"Cryo-EM studies of microtubule-MAP interactions in vitro and in situ"
Andreas Hoenger, PhD
Dept of Molecular, Cellular and Developmental Biology
University of Colorado at Bolder
Happy Hour to follow
Light Sheet Microscopy
TAC building auditorium N107
Wednesday June 3rd
2:00–3:00 PM"Sharper, faster, deeper, gentler: 4D imaging technologies for interrogating live cells and embryos"
Hari Schroff, PhD
Section of High Resolution Imaging
NIH/NIBIB
3:00–3:30 PM"Towards the living connectome: Systematic single cell analysis of neurodevelopment"
Daniel Colon-Ramos, PhD
Dept of Cell Biology
Yale University
Coffee break
4:00–5:00 PM"Lattice light sheet microscopy: imaging molecules, cells, and embryos at high spatiotemporal resolution"
Wesley Legant, PhD
Optical Physics
HHMI Janelia Farm, Ashburn VA
Happy Hour to follow

II) Equipment, Software and Technical Demonstrations and Lectures

Tuesday, June 2nd
9:00–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis
Imaris (TAC N213)
Volocity (TAC N11)
Amira and Avizo (TAC N209)
9:00–5:00 PMContinued access to most instrumentation throughout the day
TAC N221 - N239
9:15–10:00 AM"Fundamentals of confocal microscopy"
Technical lecture TAC N203
Joerg Bewersdorf, Yale University
10:45–11:30 AMDemo of SEM sample prep w Leica CPD300 Critical Point Dryer system
Leica demo TAC N231
11:00 AM–12:00 PMYale EM facility Tour
SHM building IE-26
11:30 AM–12:15 PMDemo of JEOL Neoscope for benchtop SEM
JEOL/Nikon demonstration TAC N221
12:15–1:00 PM"Imaris XT & Imaris Open: Advanced image analysis enabling custom scripts within an expert forum"
Imaris lecture TAC N213
1:00–2:00 PM"In vivo and cleared tissue imaging with the Zeiss Lightsheet Z.1"
Scott Olenych PhD, Zeiss product manager TAC N203
Wednesday, June 3rd
9:00–5:00 PMContinued access to most instrumentation throughout the day
TAC N221 - N239
9:15–9:45 AM"Light Sheet/SPIM microscope design considerations"
Thomas Pingle, LaVision BioTec technical lecture TAC N203
9:45–10:15 AM"Sample Preparation of Thick Tissues for Light Sheet imaging"
TBD, ___ technical lecture TAC N203
11:00–12:00 AMLeica EM HPM100 high pressure freezer and transfer demo
EM cryo sample preparation demo SHM building IE-40
12:00–1:00 PMTBD
12:30–1:30 PM"Amira & Avizo software for visualization, analysis, & simulation with 3D light & EM data"
FEI/VSG lecture TAC N209
1:00–2:00 PMTBD
12:30–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis
Imaris (TAC N213)
Volocity (TAC N211)
Amira and Avizo (TAC N209)
Thursday, June 4th
9:00–5:00 PMContinued access to most instrumentation throughout the day
TAC N2221 - N239
10:00–11:00 AMFEI Vitrobot vitrification robot demonstration
FEI demonstration in TAC N237
10:00–11:00 AMDemo of Lavision BioTec two-photon laser scanning microscope
Staff demonstration in TAC S614
11:00 AM–12:00 PMYale EM facility Tour
SHM building IE-26
12:30–5:00 PMSoftware Walk-in Clinics for post acquisition image analysis
Imaris (TAC N213)
Volocity (TAC N211)
Amira and Avizo (TAC N209)
1:00–2:00 PMDemo of Leica Ti:Sapphire STED microscope
Lecia demonstration in SHM IEwing
1:00–2:00 PM"Collaborative Opportunities with the Leica Center of Excellence at Yale"
Doug Giszczynski, Leica Regional Manager, in TAC N203
2:00–2:30 PM"High Throughput Fluorescence Microscopy at the Yale Center for Molecular Discovery: Rapid Methods for Optimizing Your Immunostaining Protocol and Quantifying Images in 96- and 384-well Plates"
Yulia Surovtseva, PhD and Janie Merkel, PhD, Yale Center for Molecular Discovery
3:00–5:00 PMTour of Leica Center of Excellence on Yale's West Campus (shuttle required)
Validimir Polejaev, Director Leica Center of Excellence

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.
Tuesday, June 2nd
10:30 AM–12:00 PMSmall group class at confocals: "Optimizing confocal imaging"
(for group #1)
TAC N221 - N229
2:00–5:00 PMOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use
Wednesday, June 3rd
10:30 AM–12:00 PMSmall group hands-on class at Light Sheet microscopes
(for group #2)
TAC N221 - N229
2:00–5:00 PMOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use
Thursday, June 4th
10:30 AM–12:00 PMSmall group hands-on class at Light Sheet microscopes
(for group #3)
TAC N221 - N229
10:30 AM–12:00 PMSmall group class at confocals: "Optimizing confocal imaging"
(for group #3)
TAC N221 - N229
2:00–5:00 PMOpportunity for individual practice - please reseve an instrument through the online scheduling system for guided use

Equipment List

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  • Gatan high sensitivity camera for EM
    available for demonstration in Yale EM facility

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  • LaVision BioTec Light Sheet Ultramicroscope
    Can be reserved for 1.5 hr. Available in TAC building N239 during the workshop
  • Two-photon laser scanning microscope with NDD PMTs
    outfitted with Chameleon Vision II laser (see above; can be reserved for 1 hr)

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  • Olympus FV1200 confocal
    Can be reserved for 1 hr

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  • The Zeiss Light sheet microscope will not be installed on the Yale campus, but can be viewed at the Zeiss center in Thornwood, NY which is 1.5 hours away by car.
    Individuals interested in seeing a demonstration of this instrument at the Thornwood center should contact Geoff Guimaraes directly via email (geoff.guimaraes@zeiss.com).
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Featured Software for Post Acquisition Image Analysis


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  • Gatan 3view software
    3D rendering and quantiative measurement software suitable for analysis of serial block-face EM and correlative microscopy

Featured Accessories

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Molecular Probes / Invitrogen



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Warner Instruments