2012

2012 Schedule of Events

Listed below within separate sections:

I ) Symposium
II) Equipment, software and technical demonstrations
III) Hands-on practicals 

I) Symposium Schedule

Optogenetics - Part I
TAC building auditorium N107
Tuesday, June 26th

 
2:00 – 3:00 PM

"Conditional control of protein activity using optical dimerizers" 

Chandra Tucker , PhD
Department of Pharmacology 
University of Colorado, Denve

3:00 – 3:30 PM

"Acute and local manipulations of plasma membrane phosphoinositides"

Olof Hagren, PhD 
Department of Cell Biology 
Yale University 

Coffee break 
4:00 – 5:00 PM"Peeking and poking at RhoGTPase networks in living systems"   

 Klaus Hahn, Ph.D.
Department of Pharmacology 
University of North Carolina  
 

Happy Hour to follow 
  
Optogenetics - Part II
Wednesday June 27th 
 
2:00 – 3:00 PM  "Imaging voltage with microbial rhodopsins"

Adam Cohen, PhD
Department of Chemistry & Chemical Biology, Physics 
Harvard University 

 3:00 – 3:30 PM

"Combining optogenetics and 2-photon microscopy to reveal inhibitory control of dendritic signaling."

Chiayu Chiu, PhD 
Department of Neurobiology 
Yale University 

Coffee break 
4:00 – 5:00 PM

"Do-it-yourself optogenetics in freely behaving worms"

Hang Lu, PhD
Chemical and Biomolecular Engineering 
Georgia Institute of Technology

Happy Hour to follow 

II) Equipment, Software and Technical Demonstrations 


Tuesday, June 26th
 
 9:00 – 5:00 PM

Software Walk-in Clinics for post acquisition image analysis

Imaris (TAC N205)
Volocity (TAC N09) 
TissueQuest, HistoQuest & HemoQuest (TAC N213)

9:00 – 5:00 PM 

Continued access to most instrumentation throughout the day

TAC N221 - N239

11:30 – 12:00 PM

Presentation on CYTOO micropatterned substrates for normalization of cell morphology & 
control of cytoskeleton & organelle positioning 

CYTOO lecture FMB 201

12:15 – 1:00 PM "Emerging camera technologies: a guide to CCDs and sCMOS cameras" 

Hamamatsu lecture FMB 201

1:00 – 2:00 PMDemo of Leica Ti:Sapphire STED microscope 

Lecia demonstration in SHM IE wing

1:00 – 2:00 PMSpinning Disk Demonstration  

BioVision CARV II spinning disk demonstration TAC N239

  
Wednesday, June 27th 
9:00 – 5:00 PM

Software Walk-in Clinics for post acquisition image analysis

Imaris (TAC N205)


Volocity (TAC N209) 
TissueQuest, HistoQuest & HemoQuest (TAC N213)

9:00 – 5:00 PM

Continued access to most instrumentation throughout the day

TAC N221 - N239

11:00 – 11:30 AM

Presentation on Columbus software for remote high volume image management & analysis 

Perkin Elmer lecture and demonstration TAC N209

12:30 – 2:00 PM Demo of Leica Ground State Depletion super resolution microscope 

Lecia demonstration in TAC N221

1:00 – 2:00 PM  "Basic fluorescence widefield deconstructed: understanding the building blocks of  fluorescence microscopes " 

Nikon interactive session in TAC N223

  
Thursday, June 28th 
 9:00 - 12 PM

Software Walk-in Clinics for post acquisition image analysis

Imaris (TAC N205) 
Volocity (TAC N209)

9:00 - 5:00 PM  

Continued access to most instrumentation throughout the day

TAC N2221 - N239  

1:00 – 1:30 PM

Demo of InjectMan NI2 programable micromanipulator 

Eppendorf demo in TAC N223

1:00 – 2:00 PM

"Latest Confocal Technologies from Leica" 

Leica presentation in TAC N221

2:00 – 3:00 PM

TIRF demonstration

Lecia demonstration in TAC N221

2:30 – 3:00 PM

Presentation: TissueGnostics integrated "image to data" system for whole slide image analysis 

TissueGnostics demo in TAC N239

3:30 – 4:30 PM   

Demo of Lavision BioTec two-photon laser scanning microscope

Staff demonstration in TAC S614

III) Practical Micro-Courses

Opportunity for individual practice - please reserve an instrument for personal use
Wednesday, June 27th 
10:30 AM–12:00 PM    

Small group class at confocals: photoinduction of an optogenetic protein with confocal laser scanning microscopes (for group #2)

TAC N221 - N229

2:00 – 5:00 PM 
  
Thursday, June 28th 
10:30 AM–12:00 PM

Small group class at confocals: photoinduction of an optogenetic protein with confocal laser scanning microscopes (for group #3)

TAC N221 - N229

2:00 – 5:00 PM

Equipment List

LaVision BioTec

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Olympus

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ZEISS

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  • LSM 710 spectral confocal for 3D, multicolor fluorescence imaging of any sample
  • Axio Zoom.V16 for high res, brilliant fluorescence imaging of large samples
  • Axio Vert.A1 for multiple contrast techniques in one easy-to-use, inverted stand

Featured Software for Post Acquisition Image Analysis

Volocity

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BITPLANE Scientific Solutions

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BioVision Technologies: MetaMorph Software

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  • MetaMorph software 
    Flexible and powerful tools for the image acquisition, processing, and analysis required for demanding live-cell imaging needs.

TissueGnostics Software

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  • TissueGnostics software 
    TissueQuest, HistoQuest & HemoQuest softwares for cell, tissue or whole slide acquisition, processing & formalized quantitative reporting.

Featured Accessories

Molecular Probes / Invitrogen

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Molecular Probes / Invitrogen

CYTOO Micropatterned Substrates

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CYTOO micropatterned substrates
  • micropatterned cell culture substrates for normalization of cell morphology & control of cytoskeleton & organelle positioning.

Hamamatsu PMTs, CCDs, and sCMOS Cameras

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Warner Instruments

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Warner Instruments
Click the image above to view the entire poster.