2008

2008 Schedule of Events

Listed below within separate sections:

I ) Symposium Schedule
II) Classes, Hands-on Practicals, and Technical Demonstrations
III) Equipment and Software Demonstrations

I) Symposium Schedule

TAC building auditorium N107
Tuesday June 10th FRET FOCUS
9:30 – 10:30 AM“Dissection Ca2+ and phosphoinositide signaling pathways using chemical perturbations 
and live-cell imaging”

Tobias Meyer, Ph.D.
Department of Chemical and Systems Biology
Stanford University
Coffee break
11:00 –12:00 "Stimulated calcium dynamics in mast cells: space, time, and molecular interactions"
Barbara Baird, Ph.D.
Division of Chemistry and Chemical Biology
Cornell University
Wednesday June 11th 3D AND 4D IMAGING
9:30 – 10:30AM 3D and 4D imaging of zebrafish visual system structure and development”
Chi-Bin Chien, Ph.D.
Department of Neurobiology and Anatomy 
University of Utah Medical Center
Coffee break
11:00 – 12:00 “From SAP-less T cells to helpless B cells and back: 2-photon illumination of 
dynamic cell cooperation underlying the formation of germinal centers”

Hai Qi, Ph.D.
Division of Immunology
NIH, NIAID

II) Classes, Hands-on Practicals, and Technical Demonstrations

Tuesday June 10th
1:00 – 2:00 PM  "Molecular imaging using FRET microscopy: a technical lecture and application 
in innate immunity research"

Eicke Latz, M.D. Ph.D.
Division of Infectious Diseases and Immunology 
University of Massachusetts Medical School 
TAC building room N203
2:00 – 4:00 PMFRET hands-on practical
with confocals from all major vendors
TAC N221 - N229
4:00 – 5:00 PM  TIRF Demonstration
Olympus multi-angle and color TIRF TAC N223
4:00 – 5:00 PM Approaches to Live Cell Imaging
Nikon demo of Biostation IM, A1 confocal and TIRF TAC N221
Wednesday June 11th
1:00 – 2:00 PM "Approaches to optical sectioning of tissue: confocal and two-photon 
laser scanning microscopy and other emerging techniques"

Michael Levene, Ph.D.
Division of Biomedical Engineering
Yale University
TAC auditorium N107
2:00 – 4:00 PM  Optical sectioning hands-on practical
thick tissue samples provided as well as fixed cell slides
TAC N221 - N229
4:00 – 5:00 PM  3D rendering and deconvolution practical
post-acquisition image analysis
Imaris TAC N207
4:00 – 5:00 PM  Structured Illumination for Widefield Microscopy
Zeiss Apotome demonstration TAC N229
4:00 – 5:00 PM  Laser Microdissection Demonstration
Leica LMD demonstration TAC N231
Thursday June 12th
11:00 – 12:00 Fluorescence Lifetime Imaging (FLIM) Demonstration
LaVision Biotec TAC N237
1:00 – 3:00 PM  FRET hands-on practical (2nd cohort for Yale personnel only)
with confocals from all major vendors
TAC N225 - N229
1:00 – 3:00 PMOptical sectioning hands-on practical (2nd cohort for Yale personnel only)
thick tissue samples provided as well as fixed cell slides
TAC N221 - N223
4:00 – 5:00 PM  3D rendering and deconvolution practical
post-acquisition image analysis
Imaris TAC N207     

III) Equipment and Software Demonstrations

Tuesday June 10th
10:00 – 4:00 PMSoftware Walk-in Clinics for post acquisition image analysis
Imaris (TAC N207)
9:00 – 2:00 PM  Continued access to all instrumentation througout the day
TAC N221 - N229
Wednesday June 11th
10:00 – 4:00 PMSoftware Walk-in Clinics for post acquisition image analysis
Imaris (TAC N207)
9:00 – 2:00 PMContinued access to all instrumentation througout the day
TAC N2221 - N229
Thursday June 12th
9:00 – 2:00 PMContinued access to all instrumentation througout the day
TAC N2221 - N229
10:00 – 4:00 PMSoftware Walk-in Clinics for post acquisition image analysis
Imaris (TAC N207)

Equipment List

COHERENT

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LaVision BioTec

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Praire Technologies

  • Ultima two-photon LSM
    Placed on Newport optical table (described below) with Coherent laser (can be reserved for 2 hrs)

Zeiss

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  • Zeiss 710 spectral confocal LSM and Zen software equipped with Coherent Chameleon tunable pulsed laser
    (can be reserved for 1 hr)
  • Inverted AxiObserver w/ Apotome module for structured illumination
  • Upright AxioImager w/ Apotome module + Axiovision v4.7 software

Newport

Click the image above to view the entire poster.