2008

2008 Schedule of Events

Listed below within separate sections:

I ) Symposium Schedule
II) Classes, Hands-on Practicals, and Technical Demonstrations
III) Equipment and Software Demonstrations

I) Symposium Schedule

TAC building auditorium N107
Tuesday June 10th FRET FOCUS
9:30 – 10:30 AM“Dissection Ca2+ and phosphoinositide signaling pathways using chemical perturbations 
                                    and live-cell imaging”

                                    
Tobias Meyer, Ph.D.
                                    Department of Chemical and Systems Biology
                                    Stanford University
 Coffee break 
  11:00 –12:00  "Stimulated calcium dynamics in mast cells: space, time, and molecular interactions"
                                    Barbara Baird, Ph.D.
                                    Division of Chemistry and Chemical Biology
                                    Cornell University
  
Wednesday June 11th 3D AND 4D IMAGING 
    9:30 – 10:30 AM  3D and 4D imaging of zebrafish visual system structure and development”
                                     Chi-Bin Chien, Ph.D.
                                     Department of Neurobiology and Anatomy 
                                     University of Utah Medical Center
  Coffee break 
11:00 – 12:00   “From SAP-less T cells to helpless B cells and back: 2-photon illumination of 
                                    dynamic cell cooperation underlying the formation of germinal centers”

                                    Hai Qi, Ph.D.
                                    Division of Immunology
                                    NIH, NIAID

II) Classes, Hands-on Practicals, and Technical Demonstrations

Tuesday June 10th 
1:00 – 2:00 PM  "Molecular imaging using FRET microscopy: a technical lecture and application 
                                    in innate immunity research"

                                    Eicke Latz, M.D. Ph.D.
                                    Division of Infectious Diseases and Immunology 
                                    University of Massachusetts Medical School 
                                    TAC building room N203
2:00 – 4:00 PM FRET hands-on practical
                                    with confocals from all major vendors
                                    TAC N221 - N229
4:00 – 5:00 PM    TIRF Demonstration
                                    Olympus multi-angle and color TIRF TAC N223
4:00 – 5:00 PM Approaches to Live Cell Imaging
                                    Nikon demo of Biostation IM, A1 confocal and TIRF TAC N221
  
Wednesday June 11th 
   1:00 – 2:00 PM  "Approaches to optical sectioning of tissue: confocal and two-photon 
                                   laser scanning microscopy and other emerging techniques"

Michael Levene, Ph.D.
                                    Division of Biomedical Engineering
                                    Yale University
                                    TAC auditorium N107
2:00 – 4:00 PM  Optical sectioning hands-on practical
                                   thick tissue samples provided as well as fixed cell slides
                                    TAC N221 - N229
4:00 – 5:00 PM   3D rendering and deconvolution practical
                                    post-acquisition image analysis
                                    Imaris TAC N207
4:00 – 5:00 PM  Structured Illumination for Widefield Microscopy
                                    Zeiss Apotome demonstration TAC N229
4:00 – 5:00 PM   Laser Microdissection Demonstration
                                    Leica LMD demonstration TAC N231
  
Thursday June 12th 
    11:00 – 12:00  Fluorescence Lifetime Imaging (FLIM) Demonstration
                                    LaVision Biotec TAC N237
1:00 – 3:00 PM   FRET hands-on practical (2nd cohort for Yale personnel only)
                                    with confocals from all major vendors
                                    TAC N225 - N229
1:00 – 3:00 PMOptical sectioning hands-on practical (2nd cohort for Yale personnel only)
                                   thick tissue samples provided as well as fixed cell slides
                                    TAC N221 - N223
4:00 – 5:00 PM   3D rendering and deconvolution practical
                                    post-acquisition image analysis
                                    Imaris TAC N207     

III) Equipment and Software Demonstrations

Tuesday June 10th 
    10:00 – 4:00 PM Software Walk-in Clinics for post acquisition image analysis
                                    Imaris (TAC N207)
9:00 – 2:00 PM    Continued access to all instrumentation througout the day
                                    TAC N221 - N229
Wednesday June 11th 
    10:00 – 4:00 PMSoftware Walk-in Clinics for post acquisition image analysis
                                    Imaris (TAC N207)
9:00 – 2:00 PM   Continued access to all instrumentation througout the day
                                    TAC N2221 - N229
  
Thursday June 12th 
   9:00 – 2:00 PMContinued access to all instrumentation througout the day
                                    TAC N2221 - N229
10:00 – 4:00 PM  Software Walk-in Clinics for post acquisition image analysis
                                    Imaris (TAC N207)

Equipment List

COHERENT

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LaVision BioTec

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Praire Technologies

  • Ultima two-photon LSM
    Placed on Newport optical table (described below) with Coherent laser (can be reserved for 2 hrs)

Zeiss

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  • Zeiss 710 spectral confocal LSM and Zen software equipped with Coherent Chameleon tunable pulsed laser
    (can be reserved for 1 hr)
  • Inverted AxiObserver w/ Apotome module for structured illumination
  • Upright AxioImager w/ Apotome module + Axiovision v4.7 software

Newport

Click the image above to view the entire poster.