Frequently Asked Questions
- Is this open to the general public?
- Yes. This free event is designed to provide an open forum for emerging technology and methodology. Everyone within the research community or biomedical industry are welcome to attend.collapse
- What makes this workshop different from others?
- It is a great opportunity to engage informed instrument operators and explore new microscopy approaches to your biological questions. This event strives to provide direct access to fully functional instruments and expertise at no cost and without any major time committments. In this way, interested individuals can more easily explore new microscopy approaches to determine whether particular instruments or techniques suit their future needs. Additionally, vendor operators are available to offer advice on ongoing research projects and are more than willing to explore your personal samples with you. You would be hard-pressed to see and experience so many instruments in a single setting with the kinds of specimens that are the most meaningful to you.collapse
- Where and when is this taking place?
When: June 2nd-4th , 2015.
Where : TAC building
300 Cedar Street
(corner of Cedar and Congress Ave)
New Haven CT, USA
The afternoon symposia will take place in the TAC building auditorium (N107). The microscopy equipment will be available for use in a series of contiguous rooms on the 2nd floor directly above the auditorium (N221-N231). Some equipment in other campus buildings may also be demonstrated or available for use.collapse
- Is registration required? Is there an entrance fee?
- There are no charges associated with this event. Registration is not required for the seminars, but free registration is required to enter the rooms where the microscopes are held or to attend demonstrations, technical lectures or software walk-in clinics. Although we encourage registration online, interested individuals can also register on site in the lobby or 2nd floor landing of the TAC building each day. Applicants will then receive a username and password that will enable them to reserve specific pieces of equipment via ScheduleBook.com.collapse
- Can I really bring my own samples?
Yes. Absolutely. In fact this is STRONGLY ENCOURAGED! Where else can you see your samples on so many different instruments in the same day?! You can bring fixed or living specimens, but these must be limited to BLS1 samples. BLS2 samples are not permitted. If you need a sample dish to be held at 37 C, 5% CO2 in a nearby lab please write to one of the organizers. Please note that there will not be an incubator in the rooms housing the microscopes.
EM samples: While EM imaging can be performed with samples we have prepared that will be provided on site, if you are interested in imaging your OWN samples, they must be prepared prior to the workshop. Most EM sample preparation requires several days of processing, and hence cannot be completed from beginning to end while at the workshop. Preparation for SEM prior to the workshop is fairly straightforward, as described in these suggested protocols. You merely need to pass your sample through the fixative solutions and then while at the workshop, sample preparation can be completed using the Leica CDP300 instrument that will be available to complete the dehydration and metal coating needed for SEM. Then take it to the JEOL Neoscope to obtain SEM images! Preparation for TEM is more complex as it requires fixation followed by treatment with hazardous chemicals over the course of several days. For those investigators at Yale, fixative solutions can be obtained from the Yale EM facility staff that will allow you to fix your samples as described in these protocols for tissues or cultured cells. The EM facility has generously offered to complete the labeling process for you, as they cannot provide the hazardous chemicals required for the remaining steps BUT preparations for this must be arranged in advance. Last minute requests may not be possible. Please contact Xinran Liu (firstname.lastname@example.org) with any further questions about EM sample preparation.
Super Resolution samples: Many of the super resolution microscopes have restrictions in the fluorophores that can be used. Attendees are strongly advised to follow manufacturer protocols for sample preparation. Protocols can be downloaded from the links below.collapse
- How do I reserve time on an instrument? Must I make a reservation to view the equipment?
Although entrance to the rooms and viewing of the microscopy equipment does not require a reservation, hands-on access to some of the equipment can not be guaranteed without advance reservation. Most of the time, these microscopes will be available for use outside of class times, but if you would like to guarantee access to a particular instrument you should reserve time on it through our online scheduling system.
Registered individuals will receive by email a username and password that can be used to reserve equipment on the website ScheduleBook.com. After logging onto the site in the lower right hand corner of the homepage, registrants can reserve a specific piece of equipment by first choosing the preferred date by clicking on the "pick date" button in the upper right hand quadrant of the screen. Then click on the “event” button in the upper right hand quadrant of the screen. Please record your cell phone number in the comments box so that we may contact you to discuss the reservation if necessary. Reservations can be edited or deleted by clicking on the small “E” or “D” next to your name within the time slot.
Although you may reserve up to 4 different pieces of equipment, we request that reservations be kept to a minimum in order to allow as many individuals access to the equipment as possible. Out of respect for others, please delete your reservations as soon as you know that you will not need them.collapse
- Can I really try out EM, or two photon LSM, or fancy image analysis?
Representatives from each of the microscope companies will be available to guide interested individuals in performing any technique that is applicable to the available equipment and samples. Of course you are welcome to bring any BSL1 sample with you that would suit your application of interest! The samples provided at this year's workshop will be appropriate for traditional confocal, super resolution and spinning disk microscopy, but these preparations would not be very interesting for FRET or time resolved imaging. Thick fixed specimens will be available at the LaVision BioTec two-photon LSM.
Prepared samples will be available at the scanning and transmission EM microscopes as well. If you would like to bring your own samples, please note that sample preparation for TEM and SEM requires several days of processing. Please see the section on "Can I really bring my own samples?" for a discussion of sample preparation.
Post acquisition image analysis is also available during software "walk-in clinics". Vendor representatives for Imaris, Volocity and Amira and Avizo work with you to process either your own data or exemplary data sets that they can provide to demonstrate 3D visualization, cell tracking and tomographic analyses.collapse
- Is lunch provided?
- No, but there are typically over 15 vending carts on the street within one block of the workshop that offer a wide variety of American and ethnic foods, including Thai, Ethiopian, Vietnamese, Mexican and of course Chinese. It is such a diverse collection of cuisines that it was even written up in the New Times food section. We will give you coffee and pastries though!collapse
- How do I get to the TAC building? Were do I park my car?
Visit the following website for the location of the TAC building and suggested parking within the medical campus:
http://www.yale.edu/about/maps.html - For additional maps and resourcescollapse
- What companies are participating
- Leica, Nikon, LaVision BioTec, FEI, and JOEL will all be bringing microscopes. Coherent will be providing a two-photon laser. Post acquisition image analysis software from Bitplane (Imaris), Improvison (Volocity), and VSG (Amira and Avizo) will be available as well. Please go to the <a href="http://microscopy.med.yale.edu/equipment.html">"Available Equipment"</a> and <a href="http://microscopy.med.yale.edu/software.html">"Featured Software"</a> links to see a complete list.