We (and others) have shown that persistent double-stranded DNA breaks (DSBs) associate with the nuclear periphery. We are currently investigating the signals and biochemical interactions that drive this association as well as the biological consequence of coupling DSBs to the LINC complex.
Chromatin and Nuclear Envelope Structure
In order to investigate how chromatin contributes to nuclear mechanics in a quantitative manner, we are establishing methods to apply force spectroscopy to isolated S. pombe nuclei through a collaboration with the laboratory of Simon Mochrie Ph.D. (Physics). We can manipulate isolated nuclei, associate them with a glass surface and bind beads to the nuclei. Using an optical trap, we can induce repeated rounds of compression and extension and extract quantitative information about nuclear elasticity. This assay is being applied to nuclei with different chromatin to nuclear volume ratios and perturbations in chromatin-nuclear envelope attachments.
Interface of LINC Complexes and Microtubules
A. Adapters between KASH proteins and microtubules
B. Additional membrane proteins
C. Adapters between Ima1 and chromatin
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- 7-181198972e31afb98842a68ffcaf296e471443.mp4rtmp://stream.technolutions.com/vodClick on movie to pause.Dividing S. pombe with GFP labeled Cut11, spindle...Dividing S. pombe with GFP labeled Cut11, spindle pole body docking protein.Click on movie to pause.(00:05)
- 7-181195c8a8b41e76b1420381c05861583c9663.mp4rtmp://stream.technolutions.com/vodClick on movie to pause.S. pombe during meiosis.Click on movie to pause.(00:30)