Publications

Podolski M, Mahamdeh M and Howard J: Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency. J Biol Chem. 2014 Aug 29; Epub 2014 Aug 29. PMID: 25172511


Howard J: Jonathon Howard: motor proteins go walkabout. Interview by Caitlin Sedwick. J Cell Biol. 2014 Jan 20;204 (2) :150-1. PMID: 24446480

Howard J, McIntosh JR and Odde DJ: A brief scientific biography of Prof. Alan J. Hunt. Cell Mol Bioeng. 2013 Dec;6 (4) :356-360. PMID: 24683426


Takeshita N, Mania D, Herrero S, Ishitsuka Y, Nienhaus GU, Podolski M, Howard J and Fischer R: The cell-end marker TeaA and the microtubule polymerase AlpA contribute to microtubule guidance at the hyphal tip cortex of Aspergillus nidulans to provide polarity maintenance. J Cell Sci. 2013 Dec 1;126 (Pt 23) :5400-11. Epub 2013 Oct 7. PMID: 24101725


Geyer VF, Jülicher F, Howard J and Friedrich BM: Cell-body rocking is a dominant mechanism for flagellar synchronization in a swimming alga. Proc Natl Acad Sci U S A. 2013 Nov 5;110 (45) :18058-63. Epub 2013 Oct 21. PMID: 24145440


Trushko A, Schäffer E and Howard J: The growth speed of microtubules with XMAP215-coated beads coupled to their ends is increased by tensile force. Proc Natl Acad Sci U S A. 2013 Sep 3;110 (36) :14670-5. Epub 2013 Aug 20. PMID: 23964126


Reber SB, Baumgart J, Widlund PO, Pozniakovsky A, Howard J, Hyman AA and Jülicher F: XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules. Nat Cell Biol. 2013 Sep;15 (9) :1116-22. Epub 2013 Aug 25. PMID: 23974040


Jannasch A, Bormuth V, Storch M, Howard J and Schäffer E: Kinesin-8 is a low-force motor protein with a weakly bound slip state. Biophys J. 2013 Jun 4;104 (11) :2456-64. PMID: 23746518


Zanic M, Widlund PO, Hyman AA and Howard J: Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels. Nat Cell Biol. 2013 Jun;15 (6) :688-93. Epub 2013 May 12. PMID: 23666085


Alper JD, Tovar M and Howard J: Displacement-weighted velocity analysis of gliding assays reveals that Chlamydomonas axonemal dynein preferentially moves conspecific microtubules. Biophys J. 2013 May 7;104 (9) :1989-98. PMID: 23663842


Liang X, Madrid J, Gärtner R, Verbavatz JM, Schiklenk C, Wilsch-Bräuninger M, Bogdanova A, Stenger F, Voigt A and Howard J: A NOMPC-dependent membrane-microtubule connector is a candidate for the gating spring in fly mechanoreceptors. Curr Biol. 2013 May 6;23 (9) :755-63. Epub 2013 Apr 11. PMID: 23583554


Bowne-Anderson H, Zanic M, Kauer M and Howard J: Microtubule dynamic instability: a new model with coupled GTP hydrolysis and multistep catastrophe. Bioessays. 2013 May;35 (5) :452-61. Epub 2013 Mar 27. PMID: 23532586


Gardner MK, Zanic M and Howard J: Microtubule catastrophe and rescue. Curr Opin Cell Biol. 2013 Feb;25 (1) :14-22. Epub 2012 Oct 22. PMID: 23092753


Alper J, Geyer V, Mukundan V and Howard J: Reconstitution of flagellar sliding. Methods Enzymol. 2013;524 :343-69. PMID: 23498749

Friel CT and Howard J: Coupling of kinesin ATP turnover to translocation and microtubule regulation: one engine, many machines. J Muscle Res Cell Motil. 2012 Dec;33 (6) :377-83. Epub 2012 Mar 24. PMID: 22447431


Leduc C, Padberg-Gehle K, Varga V, Helbing D, Diez S and Howard J: [How to avoid the formation of molecular motors traffic jam during intracellular transport]. Med Sci (Paris). 2012 Dec;28 (12) :1064-6. Epub 2012 Dec 21. PMID: 23290405


Widlund PO, Podolski M, Reber S, Alper J, Storch M, Hyman AA, Howard J and Drechsel DN: One-step purification of assembly-competent tubulin from diverse eukaryotic sources. Mol Biol Cell. 2012 Nov;23 (22) :4393-401. Epub 2012 Sep 19. PMID: 22993214


Senthilan PR, Piepenbrock D, Ovezmyradov G, Nadrowski B, Bechstedt S, Pauls S, Winkler M, Möbius W, Howard J and Göpfert MC: Drosophila auditory organ genes and genetic hearing defects. Cell. 2012 Aug 31;150 (5) :1042-54. PMID: 22939627


Bormuth V, Nitzsche B, Ruhnow F, Mitra A, Storch M, Rammner B, Howard J and Diez S: The highly processive kinesin-8, Kip3, switches microtubule protofilaments with a bias toward the left. Biophys J. 2012 Jul 3;103 (1) :L4-6. PMID: 22828351


Leduc C, Padberg-Gehle K, Varga V, Helbing D, Diez S and Howard J: Molecular crowding creates traffic jams of kinesin motors on microtubules. Proc Natl Acad Sci U S A. 2012 Apr 17;109 (16) :6100-5. Epub 2012 Mar 19. PMID: 22431622


Segal M, Soifer I, Petzold H, Howard J, Elbaum M and Reiner O: Ndel1-derived peptides modulate bidirectional transport of injected beads in the squid giant axon. Biol Open. 2012 Mar 15;1 (3) :220-31. Epub 2012 Jan 6. PMID: 23213412


Tropini C, Roth EA, Zanic M, Gardner MK and Howard J: Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues. PLoS One. 2012;7 (1) :e30103. Epub 2012 Jan 17. PMID: 22272281

Gardner MK, Zanic M, Gell C, Bormuth V and Howard J: Depolymerizing kinesins Kip3 and MCAK shape cellular microtubule architecture by differential control of catastrophe. Cell. 2011 Nov 23;147 (5) :1092-103. PMID: 22118464


Alper J and Howard J: Hybrid four-headed myosin motor engineered with antagonistic motor domains. Proc Natl Acad Sci U S A. 2011 Sep 20;108 (38) :15663-4. Epub 2011 Sep 7. PMID: 21900606


Friel CT and Howard J: The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization. EMBO J. 2011 Aug 26;30 (19) :3928-39. Epub 2011 Aug 26. PMID: 21873978


Gardner MK, Charlebois BD, Jánosi IM, Howard J, Hunt AJ and Odde DJ: Rapid microtubule self-assembly kinetics. Cell. 2011 Aug 19;146 (4) :582-92. PMID: 21854983


Howard J, Grill SW and Bois JS: Turing's next steps: the mechanochemical basis of morphogenesis. Nat Rev Mol Cell Biol. 2011 Jun;12 (6) :392-8. PMID: 21602907


Widlund PO, Stear JH, Pozniakovsky A, Zanic M, Reber S, Brouhard GJ, Hyman AA and Howard J: XMAP215 polymerase activity is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region. Proc Natl Acad Sci U S A. 2011 Feb 15;108 (7) :2741-6. Epub 2011 Jan 31. PMID: 21282620


Liang X, Madrid J, Saleh HS and Howard J: NOMPC, a member of the TRP channel family, localizes to the tubular body and distal cilium of Drosophila campaniform and chordotonal receptor cells. Cytoskeleton (Hoboken). 2011 Jan;68 (1) :1-7. PMID: 21069788


Bormuth V, Zörgibel F, Schäffer E and Howard J: Functional surface attachment in a sandwich geometry of GFP-labeled motor proteins. Methods Mol Biol. 2011;778 :11-8. PMID: 21809197


Friel CT, Bagshaw CR and Howard J: Analysing the ATP turnover cycle of microtubule motors. Methods Mol Biol. 2011;777 :177-92. PMID: 21773929


Gell C, Friel CT, Borgonovo B, Drechsel DN, Hyman AA and Howard J: Purification of tubulin from porcine brain. Methods Mol Biol. 2011;777 :15-28. PMID: 21773918


Mayr MI, Storch M, Howard J and Mayer TU: A non-motor microtubule binding site is essential for the high processivity and mitotic function of kinesin-8 Kif18A. PLoS One. 2011;6 (11) :e27471. Epub 2011 Nov 10. PMID: 22102900

Khairy K, Foo J and Howard J: Shapes of Red Blood Cells: Comparison of 3D Confocal Images with the Bilayer-Couple Model. Cell Mol Bioeng. 2010 Sep 1;1 (2-3) :173-181. PMID: 21031149


Redemann S, Pecreaux J, Goehring NW, Khairy K, Stelzer EH, Hyman AA and Howard J: Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos. PLoS One. 2010 Aug 20;5 (8) :e12301. Epub 2010 Aug 20. PMID: 20808841


Sanhaji M, Friel CT, Kreis NN, Krämer A, Martin C, Howard J, Strebhardt K and Yuan J: Functional and spatial regulation of mitotic centromere-associated kinesin by cyclin-dependent kinase 1. Mol Cell Biol. 2010 Jun;30 (11) :2594-607. Epub 2010 Apr 5. PMID: 20368358


Nitzsche B, Bormuth V, Bräuer C, Howard J, Ionov L, Kerssemakers J, Korten T, Leduc C, Ruhnow F and Diez S: Studying kinesin motors by optical 3D-nanometry in gliding motility assays. Methods Cell Biol. 2010;95 :247-71. PMID: 20466139


Gell C, Bormuth V, Brouhard GJ, Cohen DN, Diez S, Friel CT, Helenius J, Nitzsche B, Petzold H, Ribbe J, Schäffer E, Stear JH, Trushko A, Varga V, Widlund PO, Zanic M and Howard J: Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy. Methods Cell Biol. 2010;95 :221-45. PMID: 20466138

Kamal MM, Mills D, Grzybek M and Howard J: Measurement of the membrane curvature preference of phospholipids reveals only weak coupling between lipid shape and leaflet curvature. Proc Natl Acad Sci U S A. 2009 Dec 29;106 (52) :22245-50. Epub 2009 Dec 23. PMID: 20080790


Zanic M, Stear JH, Hyman AA and Howard J: EB1 recognizes the nucleotide state of tubulin in the microtubule lattice. PLoS One. 2009 Oct 23;4 (10) :e7585. Epub 2009 Oct 23. PMID: 19851462


Varga V, Leduc C, Bormuth V, Diez S and Howard J: Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization. Cell. 2009 Sep 18;138 (6) :1174-83. PMID: 19766569


Bormuth V, Varga V, Howard J and Schäffer E: Protein friction limits diffusive and directed movements of kinesin motors on microtubules. Science. 2009 Aug 14;325 (5942) :870-3. PMID: 19679813


Howard J and Hyman AA: Growth, fluctuation and switching at microtubule plus ends. Nat Rev Mol Cell Biol. 2009 Aug;10 (8) :569-74. Epub 2009 Jun 10. PMID: 19513082


Howard J: Mechanical signaling in networks of motor and cytoskeletal proteins. Annu Rev Biophys. 2009;38 :217-34. PMID: 19416067

Crevenna AH, Madathil S, Cohen DN, Wagenbach M, Fahmy K and Howard J: Secondary structure and compliance of a predicted flexible domain in kinesin-1 necessary for cooperation of motors. Biophys J. 2008 Dec;95 (11) :5216-27. Epub 2008 Sep 5. PMID: 18775962


Bechstedt S and Howard J: Hearing mechanics: a fly in your ear. Curr Biol. 2008 Sep 23;18 (18) :R869-70. PMID: 18812084


Bormuth V, Jannasch A, Ander M, van Kats CM, van Blaaderen A, Howard J and Schäffer E: Optical trapping of coated microspheres. Opt Express. 2008 Sep 1;16 (18) :13831-44. PMID: 18772994


Khairy K and Howard J: Spherical harmonics-based parametric deconvolution of 3D surface images using bending energy minimization. Med Image Anal. 2008 Apr;12 (2) :217-27. Epub 2007 Oct 30. PMID: 18055248


Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J and Hyman AA: XMAP215 is a processive microtubule polymerase. Cell. 2008 Jan 11;132 (1) :79-88. PMID: 18191222

Elie-Caille C, Severin F, Helenius J, Howard J, Muller DJ and Hyman AA: Straight GDP-tubulin protofilaments form in the presence of taxol. Curr Biol. 2007 Oct 23;17 (20) :1765-70. Epub 2007 Oct 4. PMID: 17919908


Riedel-Kruse IH, Hilfinger A, Howard J and Jülicher F: How molecular motors shape the flagellar beat. HFSP J. 2007 Sep;1 (3) :192-208. PMID: 19404446


Leduc C, Ruhnow F, Howard J and Diez S: Detection of fractional steps in cargo movement by the collective operation of kinesin-1 motors. Proc Natl Acad Sci U S A. 2007 Jun 26;104 (26) :10847-52. Epub 2007 Jun 14. PMID: 17569782


Howard J and Tolić-Nørrelykke IM: Bundling, sliding, and pulling microtubules in cells and in silico. HFSP J. 2007 May;1 (1) :11-4. Epub 2007 May 21. PMID: 19404456


Bormuth V, Howard J and Schäffer E: LED illumination for video-enhanced DIC imaging of single microtubules. J Microsc. 2007 Apr;226 (Pt 1) :1-5. PMID: 17381703


Schäffer E, Nørrelykke SF and Howard J: Surface forces and drag coefficients of microspheres near a plane surface measured with optical tweezers. Langmuir. 2007 Mar 27;23 (7) :3654-65. Epub 2007 Feb 28. PMID: 17326669


Howard J and Hyman AA: Microtubule polymerases and depolymerases. Curr Opin Cell Biol. 2007 Feb;19 (1) :31-5. Epub 2006 Dec 20. PMID: 17184986


Bechstedt S and Howard J: Models of hair cell mechanotransduction. Curr Top Membr. 2007;59 :399-424. Epub 2007 Apr 17. PMID: 25168144

Pecreaux J, Röper JC, Kruse K, Jülicher F, Hyman AA, Grill SW and Howard J: Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Curr Biol. 2006 Nov 7;16 (21) :2111-22. PMID: 17084695


Kerssemakers J, Howard J, Hess H and Diez S: The distance that kinesin-1 holds its cargo from the microtubule surface measured by fluorescence interference contrast microscopy. Proc Natl Acad Sci U S A. 2006 Oct 24;103 (43) :15812-7. Epub 2006 Oct 11. PMID: 17035506


Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU and Howard J: Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol. 2006 Sep;8 (9) :957-62. Epub 2006 Aug 13. PMID: 16906145


Dinu CZ, Opitz J, Pompe W, Howard J, Mertig M and Diez S: Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules. Small. 2006 Aug;2 (8-9) :1090-8. PMID: 17193173


Howard J: Protein power strokes. Curr Biol. 2006 Jul 25;16 (14) :R517-9. PMID: 16860722


Helenius J, Brouhard G, Kalaidzidis Y, Diez S and Howard J: The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends. Nature. 2006 May 4;441 (7089) :115-9. PMID: 16672973

Riedel IH, Kruse K and Howard J: A self-organized vortex array of hydrodynamically entrained sperm cells. Science. 2005 Jul 8;309 (5732) :300-3. PMID: 16002619


Poole K, Khairy K, Friedrichs J, Franz C, Cisneros DA, Howard J and Mueller D: Molecular-scale topographic cues induce the orientation and directional movement of fibroblasts on two-dimensional collagen surfaces. J Mol Biol. 2005 Jun 3;349 (2) :380-6. Epub 2005 Apr 9. PMID: 15890202

Jiang F, Hörber H, Howard J and Müller DJ: Assembly of collagen into microribbons: effects of pH and electrolytes. J Struct Biol. 2004 Dec;148 (3) :268-78. PMID: 15522775


Lawrence CJ, Dawe RK, Christie KR, Cleveland DW, Dawson SC, Endow SA, Goldstein LS, Goodson HV, Hirokawa N, Howard J, Malmberg RL, McIntosh JR, Miki H, Mitchison TJ, Okada Y, Reddy AS, Saxton WM, Schliwa M, Scholey JM, Vale RD, Walczak CE and Wordeman L: A standardized kinesin nomenclature. J Cell Biol. 2004 Oct 11;167 (1) :19-22. PMID: 15479732


Haeberle H, Fujiwara M, Chuang J, Medina MM, Panditrao MV, Bechstedt S, Howard J and Lumpkin EA: Molecular profiling reveals synaptic release machinery in Merkel cells. Proc Natl Acad Sci U S A. 2004 Oct 5;101 (40) :14503-8. Epub 2004 Sep 24. PMID: 15448211


Jiang F, Khairy K, Poole K, Howard J and Müller DJ: Creating nanoscopic collagen matrices using atomic force microscopy. Microsc Res Tech. 2004 Aug;64 (5-6) :435-40. PMID: 15549696


Sawhney RK and Howard J: Molecular dissection of the fibroblast-traction machinery. Cell Motil Cytoskeleton. 2004 Jul;58 (3) :175-85. PMID: 15146536


Howard J and Bechstedt S: Hypothesis: a helix of ankyrin repeats of the NOMPC-TRP ion channel is the gating spring of mechanoreceptors. Curr Biol. 2004 Mar 23;14 (6) :R224-6. PMID: 15043829


Schief WR, Clark RH, Crevenna AH and Howard J: Inhibition of kinesin motility by ADP and phosphate supports a hand-over-hand mechanism. Proc Natl Acad Sci U S A. 2004 Feb 3;101 (5) :1183-8. Epub 2004 Jan 20. PMID: 14734813

Grill SW, Howard J, Schäffer E, Stelzer EH and Hyman AA: The distribution of active force generators controls mitotic spindle position. Science. 2003 Jul 25;301 (5632) :518-21. PMID: 12881570


Hunter AW, Caplow M, Coy DL, Hancock WO, Diez S, Wordeman L and Howard J: The kinesin-related protein MCAK is a microtubule depolymerase that forms an ATP-hydrolyzing complex at microtubule ends. Mol Cell. 2003 Feb;11 (2) :445-57. PMID: 12620232

Sawhney RK and Howard J: Slow local movements of collagen fibers by fibroblasts drive the rapid global self-organization of collagen gels. J Cell Biol. 2002 Jun 10;157 (6) :1083-91. Epub 2002 Jun 10. PMID: 12058022


Vinh DB, Kern JW, Hancock WO, Howard J and Davis TN: Reconstitution and characterization of budding yeast gamma-tubulin complex. Mol Biol Cell. 2002 Apr;13 (4) :1144-57. PMID: 11950928


Diez S, Schief WR and Howard J: Molecular motors: single-molecule recordings made easy. Curr Biol. 2002 Mar 19;12 (6) :R203-5. PMID: 11909546