Mark Hochstrasser

Hickey CM, Hochstrasser M: STUbL-mediated Degradation of the Transcription Factor MATα2 Requires Degradation Elements that Coincide with Corepressor Binding Sites. Mol Biol Cell. 2015 Aug 5; 2015 Aug 5. PMID: 26246605

Li Y, Tomko RJ Jr, Hochstrasser M: Proteasomes: Isolation and Activity Assays. Curr Protoc Cell Biol. 2015 Jun 1; 2015 Jun 1. PMID: 26061243

Zattas D, Hochstrasser M: Ubiquitin-dependent protein degradation at the yeast endoplasmic reticulum and nuclear envelope. Crit Rev Biochem Mol Biol. 2015 Jan-Feb; 2014 Sep 18. PMID: 25231236

Kang H, Bradley MJ, Cao W, Zhou K, Grintsevich EE, Michelot A, Sindelar CV, Hochstrasser M, De La Cruz EM: Site-specific cation release drives actin filament severing by vertebrate cofilin. Proc Natl Acad Sci U S A. 2014 Dec 16; 2014 Dec 2. PMID: 25468977

Tomko RJ Jr, Hochstrasser M: The intrinsically disordered Sem1 protein functions as a molecular tether during proteasome lid biogenesis. Mol Cell. 2014 Feb 6; 2014 Jan 9. PMID: 24412063

Kunjappu MJ, Hochstrasser M: Assembly of the 20S proteasome. Biochim Biophys Acta. 2014 Jan; 2013 Mar 16. PMID: 23507199

Sá-Moura B, Funakoshi M, Tomko RJ Jr, Dohmen RJ, Wu Z, Peng J, Hochstrasser M: A conserved protein with AN1 zinc finger and ubiquitin-like domains modulates Cdc48 (p97) function in the ubiquitin-proteasome pathway. J Biol Chem. 2013 Nov 22; 2013 Oct 11. PMID: 24121501

Zattas D, Adle DJ, Rubenstein EM, Hochstrasser M: N-terminal acetylation of the yeast Derlin Der1 is essential for Hrd1 ubiquitin-ligase activity toward luminal ER substrates. Mol Biol Cell. 2013 Apr; 2013 Jan 30. PMID: 23363603

Tomko RJ Jr, Hochstrasser M: Molecular architecture and assembly of the eukaryotic proteasome. Annu Rev Biochem. 2013; 2013 Mar 13. PMID: 23495936

Hickey CM, Wilson NR, Hochstrasser M: Function and regulation of SUMO proteases. Nat Rev Mol Cell Biol. 2012 Dec. PMID: 23175280

Rubenstein EM, Kreft SG, Greenblatt W, Swanson R, Hochstrasser M: Aberrant substrate engagement of the ER translocon triggers degradation by the Hrd1 ubiquitin ligase. J Cell Biol. 2012 Jun 11. PMID: 22689655

Gillies J, Hochstrasser M: A new class of SUMO proteases. EMBO Rep. 2012 Apr 2; 2012 Apr 2. PMID: 22422001

Hochstrasser M, Funakoshi M: Disulfide engineering to map subunit interactions in the proteasome and other macromolecular complexes. Methods Mol Biol. 2012. PMID: 22350897

Felberbaum R, Wilson NR, Cheng D, Peng J, Hochstrasser M: Desumoylation of the endoplasmic reticulum membrane VAP family protein Scs2 by Ulp1 and SUMO regulation of the inositol synthesis pathway. Mol Cell Biol. 2012 Jan; 2011 Oct 24. PMID: 22025676

Tomko RJ Jr, Hochstrasser M: Incorporation of the Rpn12 subunit couples completion of proteasome regulatory particle lid assembly to lid-base joining. Mol Cell. 2011 Dec 23. PMID: 22195964

Kreft SG, Hochstrasser M: An unusual transmembrane helix in the endoplasmic reticulum ubiquitin ligase Doa10 modulates degradation of its cognate E2 enzyme. J Biol Chem. 2011 Jun 10; 2011 Apr 5. PMID: 21467040

Tomko RJ Jr, Hochstrasser M: Order of the proteasomal ATPases and eukaryotic proteasome assembly. Cell Biochem Biophys. 2011 Jun. PMID: 21461838

Kusmierczyk AR, Kunjappu MJ, Kim RY, Hochstrasser M: A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Nat Struct Mol Biol. 2011 May; 2011 Apr 17. PMID: 21499243

Rubenstein EM, Hochstrasser M: Redundancy and variation in the ubiquitin-mediated proteolytic targeting of a transcription factor. Cell Cycle. 2010 Nov 1; 2010 Nov 22. PMID: 20980825

Su D, Hochstrasser M: A WLM protein with SUMO-directed protease activity. Mol Cell Biol. 2010 Aug; 2010 Jun 21. PMID: 20566698

Tomko RJ Jr, Funakoshi M, Schneider K, Wang J, Hochstrasser M: Heterohexameric ring arrangement of the eukaryotic proteasomal ATPases: implications for proteasome structure and assembly. Mol Cell. 2010 May 14. PMID: 20471945

Xie Y, Rubenstein EM, Matt T, Hochstrasser M: SUMO-independent in vivo activity of a SUMO-targeted ubiquitin ligase toward a short-lived transcription factor. Genes Dev. 2010 May; 2010 Apr 13. PMID: 20388728

Funakoshi M, Tomko RJ Jr, Kobayashi H, Hochstrasser M: Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell. 2009 May 29; 2009 May 14. PMID: 19446322

Kroetz MB, Su D, Hochstrasser M: Essential role of nuclear localization for yeast Ulp2 SUMO protease function. Mol Biol Cell. 2009 Apr; 2009 Feb 18. PMID: 19225149

Hochstrasser M: Origin and function of ubiquitin-like proteins. Nature. 2009 Mar 26. PMID: 19325621

Funakoshi M, Hochstrasser M: Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae. Yeast. 2009 Mar. PMID: 19243080

Kroetz MB, Hochstrasser M: Identification of SUMO-interacting proteins by yeast two-hybrid analysis. Methods Mol Biol. 2009. PMID: 19107413

Wang L, Dong H, Soroka CJ, Wei N, Boyer JL, Hochstrasser M: Degradation of the bile salt export pump at endoplasmic reticulum in progressive familial intrahepatic cholestasis type II. Hepatology. 2008 Nov. PMID: 18798335

Kusmierczyk AR, Hochstrasser M: Some assembly required: dedicated chaperones in eukaryotic proteasome biogenesis. Biol Chem. 2008 Sep. PMID: 18713001

Kusmierczyk AR, Kunjappu MJ, Funakoshi M, Hochstrasser M: A multimeric assembly factor controls the formation of alternative 20S proteasomes. Nat Struct Mol Biol. 2008 Mar; 2008 Feb 17. PMID: 18278055

Felberbaum R, Hochstrasser M: Ulp2 and the DNA damage response: desumoylation enables safe passage through mitosis. Cell Cycle. 2008 Jan 1; 2007 Oct 28. PMID: 18196960

Xie Y, Kerscher O, Kroetz MB, McConchie HF, Sung P, Hochstrasser M: The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation. J Biol Chem. 2007 Nov 23; 2007 Sep 11. PMID: 17848550

Schwartz DC, Felberbaum R, Hochstrasser M: The Ulp2 SUMO protease is required for cell division following termination of the DNA damage checkpoint. Mol Cell Biol. 2007 Oct; 2007 Jul 30. PMID: 17664284

Lewis A, Felberbaum R, Hochstrasser M: A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillance. J Cell Biol. 2007 Aug 27. PMID: 17724121

Hochstrasser M: Ubiquitin ligation without a ligase. Dev Cell. 2007 Jul. PMID: 17609103

Li X, Kusmierczyk AR, Wong P, Emili A, Hochstrasser M: beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint. EMBO J. 2007 May 2; 2007 Apr 12. PMID: 17431397

Ravid T, Hochstrasser M: Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue. Nat Cell Biol. 2007 Apr; 2007 Feb 21. PMID: 17310239

Deng M, Hochstrasser M: Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase. Nature. 2006 Oct 19. PMID: 17051211

Arteaga MF, Wang L, Ravid T, Hochstrasser M, Canessa CM: An amphipathic helix targets serum and glucocorticoid-induced kinase 1 to the endoplasmic reticulum-associated ubiquitin-conjugation machinery. Proc Natl Acad Sci U S A. 2006 Jul 25; 2006 Jul 17. PMID: 16847254

Hochstrasser M, Cohen R: Cecile M. Pickart 1954-2006. Nat Cell Biol. 2006 Jun. PMID: 16738698

Kreft SG, Wang L, Hochstrasser M: Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI). J Biol Chem. 2006 Feb 24; 2005 Dec 22. PMID: 16373356

Ravid T, Kreft SG, Hochstrasser M: Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways. EMBO J. 2006 Feb 8; 2006 Jan 26. PMID: 16437165

Hochstrasser M: Lingering mysteries of ubiquitin-chain assembly. Cell. 2006 Jan 13. PMID: 16413479

Kerscher O, Felberbaum R, Hochstrasser M: Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu Rev Cell Dev Biol. 2006. PMID: 16753028

Hannich JT, Lewis A, Kroetz MB, Li SJ, Heide H, Emili A, Hochstrasser M: Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae. J Biol Chem. 2005 Feb 11; 2004 Dec 6. PMID: 15590687

Amerik AY, Hochstrasser M: Mechanism and function of deubiquitinating enzymes. Biochim Biophys Acta. 2004 Nov 29. PMID: 15571815

Ravid T, Hochstrasser M: NF-kappaB signaling: flipping the switch with polyubiquitin chains. Curr Biol. 2004 Oct 26. PMID: 15498483

Velichutina I, Connerly PL, Arendt CS, Li X, Hochstrasser M: Plasticity in eucaryotic 20S proteasome ring assembly revealed by a subunit deletion in yeast. EMBO J. 2004 Feb 11; 2004 Jan 22. PMID: 14739934

Laney JD, Hochstrasser M: Ubiquitin-dependent degradation of the yeast Mat(alpha)2 repressor enables a switch in developmental state. Genes Dev. 2003 Sep 15; 2003 Sep 2. PMID: 12952895

Schwartz DC, Hochstrasser M: A superfamily of protein tags: ubiquitin, SUMO and related modifiers. Trends Biochem Sci. 2003 Jun. PMID: 12826404

Li SJ, Hochstrasser M: The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity. J Cell Biol. 2003 Mar 31; 2003 Mar 24. PMID: 12654900

Laney JD, Hochstrasser M: Analysis of protein ubiquitination. Curr Protoc Protein Sci. 2002 Nov. PMID: 18429222

Hochstrasser M: Molecular biology. New proteases in a ubiquitin stew. Science. 2002 Oct 18. PMID: 12386321

Hochstrasser M: New structural clues to substrate specificity in the "ubiquitin system". Mol Cell. 2002 Mar. PMID: 11931752

Laney JD, Hochstrasser M: Assaying protein ubiquitination in Saccharomyces cerevisiae. Methods Enzymol. 2002. PMID: 12073348

Lab Member Papers

Jason Berk, Select

Berk JM, Simon DN, Jenkins-Houk CR, Westerbeck JW, Grønning-Wang LM, Carlson CR, Wilson KL: The molecular basis of emerin-emerin and emerin-BAF interactions. J Cell Sci. 2014 Sep 15; 2014 Jul 22. PMID: 25052089

Berk JM, Maitra S, Dawdy AW, Shabanowitz J, Hunt DF, Wilson KL: O-Linked β-N-acetylglucosamine (O-GlcNAc) regulates emerin binding to barrier to autointegration factor (BAF) in a chromatin- and lamin B-enriched "niche". J Biol Chem. 2013 Oct 18; 2013 Sep 6. PMID: 24014020

Berk JM, Tifft KE, Wilson KL: The nuclear envelope LEM-domain protein emerin. Nucleus. 2013 Jul-Aug; 2013 Jul 17. PMID: 23873439

Wilson KL, Berk JM: The nuclear envelope at a glance. J Cell Sci. 2010 Jun 15. PMID: 20519579

Effler JC, Kee YS, Berk JM, Tran MN, Iglesias PA, Robinson DN: Mitosis-specific mechanosensing and contractile-protein redistribution control cell shape. Curr Biol. 2006 Oct 10. PMID: 17027494

Achuth Padmanabhan, Select

Li X, Guan B, Srivastava MK, Padmanabhan A, Hampton BS, Bieberich CJ: The reverse in-gel kinase assay to profile physiological kinase substrates. Nat Methods. 2007 Nov; 2007 Oct 21. PMID: 17952089

Chisholm, C, Padmanabhan, A, Guan, B, Li, X, Kalra, S, Bieberich, CJ: Protein Kinase CK2 controls the stability of prostate derived ETS factor. The Open Cancer Journal. 2010, 3, 109-115.

Robert Tomko, Select

Li Y, Tomko RJ Jr, Hochstrasser M: Proteasomes: Isolation and Activity Assays. Curr Protoc Cell Biol. 2015 Jun 1; 2015 Jun 1. PMID: 26061243

Tomko RJ Jr, Hochstrasser M: The intrinsically disordered Sem1 protein functions as a molecular tether during proteasome lid biogenesis. Mol Cell. 2014 Feb 6; 2014 Jan 9. PMID: 24412063

Sá-Moura B, Funakoshi M, Tomko RJ Jr, Dohmen RJ, Wu Z, Peng J, Hochstrasser M: A conserved protein with AN1 zinc finger and ubiquitin-like domains modulates Cdc48 (p97) function in the ubiquitin-proteasome pathway. J Biol Chem. 2013 Nov 22; 2013 Oct 11. PMID: 24121501

Tomko RJ Jr, Hochstrasser M: Molecular architecture and assembly of the eukaryotic proteasome. Annu Rev Biochem. 2013; 2013 Mar 13. PMID: 23495936

Tomko RJ Jr, Hochstrasser M: Incorporation of the Rpn12 subunit couples completion of proteasome regulatory particle lid assembly to lid-base joining. Mol Cell. 2011 Dec 23. PMID: 22195964

Tomko RJ Jr, Hochstrasser M: Order of the proteasomal ATPases and eukaryotic proteasome assembly. Cell Biochem Biophys. 2011 Jun. PMID: 21461838

Heinemann IU, Randau L, Tomko RJ Jr, Söll D: 3'-5' tRNAHis guanylyltransferase in bacteria. FEBS Lett. 2010 Aug 20; 2010 Jul 23. PMID: 20650272

Tomko RJ Jr, Funakoshi M, Schneider K, Wang J, Hochstrasser M: Heterohexameric ring arrangement of the eukaryotic proteasomal ATPases: implications for proteasome structure and assembly. Mol Cell. 2010 May 14. PMID: 20471945

Wang P, Zou F, Zhang X, Li H, Dulak A, Tomko RJ Jr, Lazo JS, Wang Z, Zhang L, Yu J: microRNA-21 negatively regulates Cdc25A and cell cycle progression in colon cancer cells. Cancer Res. 2009 Oct 15; 2009 Oct 13. PMID: 19826040

Funakoshi M, Tomko RJ Jr, Kobayashi H, Hochstrasser M: Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell. 2009 May 29; 2009 May 14. PMID: 19446322

Tomko RJ Jr, Azang-Njaah NN, Lazo JS: Nitrosative stress suppresses checkpoint activation after DNA synthesis inhibition. Cell Cycle. 2009 Jan 15; 2009 Jan 10. PMID: 19158509

Tomko RJ Jr, Lazo JS: Multimodal control of Cdc25A by nitrosative stress. Cancer Res. 2008 Sep 15. PMID: 18794133

Queiroz de Oliveira PE, Tomko RJ Jr, Lazo JS: Is a step backwards in S-phase-targeted chemotherapy a step forward? Mol Interv. 2008 Jun. PMID: 18693192

Brisson M, Foster C, Wipf P, Joo B, Tomko RJ Jr, Nguyen T, Lazo JS: Independent mechanistic inhibition of cdc25 phosphatases by a natural product caulibugulone. Mol Pharmacol. 2007 Jan; 2006 Oct 3. PMID: 17018577

Tomko RJ Jr, Bansal P, Lazo JS: Airing out an antioxidant role for the tumor suppressor p53. Mol Interv. 2006 Feb. PMID: 16507747

Aponick A, Buzdygon RS, Tomko RJ Jr, Fazal AN, Shughart EL, McMaster DM, Myers MC, Pitcock WH Jr, Wigal CT: Regioselective organocadmium alkylations of substituted quinones. J Org Chem. 2002 Jan 11. PMID: 11777467