In vivo imaging of the neurovascular unit:
To study cell-cell interactions in the neurovascular unit we develop methods using two-photon microscopy (TPM) to repeatedly image individual neurons, dendritic spines, microglia, astrocytes and blood vessels over periods of up to months in the brain of living mice.
Alzheimer's and other neurodegenerative disorders:
We are interested in using novel imaging tools to better understand the dynamics of various neurodegenerative conditions such as Alzheimer's disease. Specifically we are interested in the interactions between microglia, neurons and amyloid plaques to determine the potential role of microglia in plaque formation, removal and secondary neuronal injury (Liu et al, J. Neurosci. 2010), (Condello et. al. Sci Rep. 2011).
Vascular mechanisms of neuronal circuit disruption:
We investigate mechanisms of microvascular recanalization and angioplasticity after cerebral embolic occlusion (Lam et al. Nature, 2010. We are also interested in understanding how neurons and astrocytes adapt to metabolic challenges such as chronic focal and global hypoperfusion and mild hypoxia. dynamics of developing microvessels (Harb et. al. JCBFM, 2012 and their interaction with perivascular cells.
Imaging cortical myelin in health and disease:
We have recently developed a methodology for in vivo microscopy of myelin in the mouse cortex. Using this and other techniques we are investigating several aspects of axonal myelination development, maintenance and degeneration.