Library Protocols

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Standard sequencing (2-6 Kb)

Standard SMRT sequencing generates single pass, long reads.

Circular consensus sequencing (250 bp)

The circular consensus sequencing protocol uses a circular DNA template to enable multiple reads across a single molecule which can increase the consensus accuracy.

Strobe sequencing (Larger 6-10 kb)

The strobe protocol increases physical coverage and effective readlength by “strobing” the illumination on and off. This technique effectively extends readlength by minimizing polymerase damage resulting from continuous laser illumination. Data is then collected at your defined illumination interval. When the illumination is off, sequencing continues at a predictable rate. This approach allows for multiple sub-reads at varying advance lengths from a single molecule.