Pacific Biosciences

General Information

Single Molecule Real-Time Analysis


Single molecule, real-time (SMRTTM) detection provides direct measurement of individual molecules, capturing multiple dimensions of data. Templates can be prepared for the PacBio RS II without PCR amplification, resulting in more uniform sequence coverage across genomic regions regardless of GC content, facilitating the detection of minor variants in heterogeneous samples. While observing single molecule sequencing by a highly processive strand-displacing DNA polymerase in real time, the system also records the kinetics of each nucleotide incorporation reaction, identifying base modifications of native templates, such as DNA methylation.

Longer Read Lengths

With read lengths in the thousands of bases, the PacBio RS II can resolve both SNPs and large-scale structural rearrangements. Long reads increase understanding of disease heritability through haplotype phasing. Long reads also simplify and improve genomic assembly by reducing the number of contigs, and produce superior consensus accuracy compared to other technologies at the same depth of coverage.

Faster Time to Results

Read DNA at the speed of the polymerase in bases per second. With rapid sample preparation completed in 4-6 hours and short run times measured in minutes, you can obtain biologically meaningful answers within hours instead of a week or more. For data analysis, we offer a robust suite of SMRT Analysis.

Flexibility

Customize your experiments with granular, low cost consumables and adjustable run conditions. Multiple sequencing protocols provide maximum flexibility for a wide range of applications.