Throughput

Performance parameters*

HiSeq

Read lengthRun time (days)# of Reads per laneFiltered data/passing Q.C. (MB/lane)
1 x 75 bp3170 million12750
1 x 100 bp4170 million17000
2 x 75 bp7340 million25500
2 x 100 bp9340 million34000
* assumes optimal cluster densities which cannot be guaranteed.

GB = gigabase, 10^9; MB = 10^6

Please use the guide here to determine how many lanes of sequencing are needed for your sample.