Pooled Exome Analysis

Sequencing costs for specific applications:
Recent R&D efforts at YCGA for analyzing pooled samples has resulted in increased throughput and significant cost savings for various applications. Since one lane of a HiSeq flowcell provides sequence for approximately 30 billion bases, it is possible to sequence multiple barcoded samples in one lane and still obtain sufficient coverage for each of the pooled samples. The following table provides costs for the most common applications. We guarantee 88% 8x coverage for pooled exome analysis. If you need further information, please contact us.

Whole-Exome Analysis:          
As indicated in the table below, the cost depends upon how many samples are pooled together.

# of barcoded exomes captured
per one oligo pool and
sequenced/lane of HiSeq flowcell

Example of
Applications/Projects

Approx. # of millions of
PE reads/exome

Cost*/exome

2

Detection of somatic variations/
Tumor samples

200

$1262

4

Identification of denovo mutations

100

$690

6

Discovery of Mendelian/
rare disease variations
and common disorders

65

$500

*Yale Prices as of 7/1/2013