Hybridization to the Chip
After the quality control assessment, 1.5 µg of cRNA is mixed with the hybridization controls and it is hybridized to the array. The array is hybridized for 16 hours in a hybridization oven with a rocking platform at 58°C. The array chip then goes through a series of washes before it is stained with streptavidin-Cy3. After the staining, it goes through a final wash and drying. The array is scanned using the Illumina BeadArray reader.