Protein Profiling
Rates as of 7/1/2012
Cost for SILAC Analysis | ||
Bundled Total Costs | Yale Cost | Non-Yale* Cost |
SILAC analysis with no fractionation - 1 sample | $1430 | $1645 |
SILAC Analysis with strong Cation fractionation and LC-MS/MS on 10 Cation fractions | $3785 | $4353 |
SILAC Analysis with 1D gel fractionation (which is done in the submitters lab) - 10 bands | $3255 | $3743 |
Individual Service | Yale Cost
| Non-Yale* Cost
|
Sample preparation (e.g. chloroform/methanol precipitation) | $115 | $132 |
manual digestion-LysC and Trypsin | $200 setup +
$60/sample | $230 setup +
$69/sample |
strong Cation exchange separation | $415 | $477 |
LC-MS/MS Run for 1 sample | $300 | $345 |
LC-MS/MS for Cation fractions or sequential gel bands (10 or more) per fraction | $170 | $196 |
SILAC Data Analysis | $695 | $799 |
ZipTip sample de-salting prior to LC-MS/MS | $60 | $69 |
*SILAC Labeling is performed in the investigators lab | ||
Cost for Proteome Lab PF2D Analysis | ||
Service | Yale Cost
| Non-Yale* Cost
|
For sample fractionation | ||
2D LC: chromatofocusing followed by non-porous reverse phase separation | $500 | $575 |
Cost for DIGE Protein Profiling Analysis | ||
Service | Yale Cost
per sample | Non-Yale* Cost
per sample |
DIGE service with Cy-labeling of 3 samples only | $872 | $1019 |
DIGE service with Cy-labeling of 2 samples only | $772 | $888 |
DIGE service with Cy-labeling of 3 samples (double sample + dye) | $1091 | $1255 |
DIGE service with Cy-labeling of 2 samples (double sample + dye) | $886 | $1019 |
Gel Scanning + Preliminary Data Analysis | $113 | $130 |
Gel Picking of up to 96 user-selected spots | $49 | $56 |
Primary Protein Identification (per spot with a 10 spot minimum; robotic digestion included) | $95 | $110 |
Immunoaffinity partitioning (depletion) of plasma/ serum samples | $447 | $514 |
Fluorescent scan on the Typhoon scanner | $40 | $46 |
DIGE Clean-up | $123 | $141 |
Pro-Q Diamond (phosoprotein stain) | $162 | $186 |
Pro-Q Emerald (glycoprotein Stain) | $162 | $186 |
| * Digestion is included | ||
Cost for iTRAQ Analysis | ||
Bundled Total Costs | Yale Cost | Non-Yale* Cost |
iTRAQ analysis 4 plex | $2610 | $3001 |
iTRAQ analysis 8 plex | $3470 | $3989 |
includes sample preparation, quality control scout runs, nanodrop protein concentration, tryptic digestion, chemical labeling and 1 x Complex Proteome LC-MS/MS. No fractionation included | ||
iTRAQ 4-plex Analysis with strong Cation fractionation and LC-MS/MS on 10 Cation fractions | $5095 | $5863 |
iTRAQ 8-plex Analysis with strong Cation fractionation and LC-MS/MS on 10 Cation fractions | $5955 | $6851 |
includes sample preparation, quality control scout runs, nanodrop protein concentration, tryptic digestion, chemical labeling, CEX fractionation and 10 x LC-MS/MS long gradient | ||
Individual Service | Yale Cost
per sample | Non-Yale* Cost
per sample |
Sample preparation (e.g. chloroform/methanol precipitation) per sample set | $115 | $132 |
nanodrop protein concentration per sample set | $115 | $132 |
manual digestion-LysC and Trypsin | $200 setup +
$60/sample | $230 setup +
$69/sample |
iTRAQ labeling (4-plex) | $805 | $926 |
iTRAQ labeling (8-plex) | $1195 | $1374 |
Complex Proteome LC-MS/MS | $500 | $575 |
ZipTip sample de-salting prior to LC-MS/MS | $60 | $69 |
iTRAQ Data Analysis | $345 | $397 |
strong Cation exchange separation- available for iTRAQ | $415 | $477 |
Profiling - Advanced iTRAQ Data Analysis | $403 | $463 |
Amino Acid Analysis quantitation also available - click here for pricing | ||
Cost for Advanced iTRAQ Statistical Analysis | ||
Service | Yale Cost
per sample | Non-Yale* Cost
per sample |
Profiling - iTRAQ Scout (30 minutes based on Consulting Rate) | $57.50 | $66 |
Adjustment - Consulting Total | $115 | $132 |
Cost for Label Free Quantitation (LFQ) Analysis | ||
Service | Yale Cost
per sample | Non-Yale* Cost
per sample |
Sample preparation (e.g. chloroform/methanol precipitation) per sample set | $115 | $132 |
nanodrop protein concentration per sample set | $115 | $132 |
manual digestion-LysC and Trypsin | $200 setup +
$60/sample | $230 setup +
$69/sample |
LC-MS/MS per run- note that triplicates are needed | $170 | $196 |
LFQ Data Analysis | $695 | $799 |
ZipTip sample de-salting prior to LC-MS/MS | $60 | $769 |
*Contact us for Non-Yale profit pricing.
