Using already established quantitative protein profiling techniques (e.g. SILAC, iTRAQ, and label-free quantitation) , relative abundance of phosphorylation between different samples can be compared. The choice of these techniques is strongly dependent on the nature of the experimental systems being used. MRM or multiple reaction monitoring can be used for absolute quantitation.
Requirements: Investigators starting on a quantitative experimental design for determining changes on level of phosphorylation are strongly encouraged to consult with Keck Staff before deciding on any experimental approaches. Similar guidelines can be followed from above on the amount of samples required to conduct quantitation experiments.