The attachment of a 16-carbon saturated fatty acid to Cys residues by a thioether bond is an important mechanism which occurs mostly in the plasma membrane and suggests the regulation of protein function. Identification of sites of palmitoylation on proteins is done through palmitoyl-enriched protein methodology coupled to an FT-ICR MS/MS protocol and/or the use of LC MS/MS via on UPLC LTQ Orbitrap MS instrument.
Roth, A.F., Wan, J., Bailey, A.O., Sun, B., Kuchar, J.A., Green, W.N., Phinney, B.S., Yates, J.R. 3rd, Davis, N.G. (2006) Global analysis of protein palmitoylation in yeast, Cell. 2006 125:1003-13.
Roth, A.F., Wan, J., Green, W.N., Yates, J.R., Davis, N.G. (2006) Proteomic identification of palmitoylated proteins. Methods. 2006 2:135-42.