General Protein Chemistry

Classical Protein Chemistry approaches of amino acid analysis (used to determine protein concentrations) and edman chemical sequencing (amino terminal protein/peptide sequencing) are still important proteomic techniques. Amino acid analysis is routinely used on samples destined for any quantitative Protein Profiling experiment, to ensure equal µg of protein are utilized in the comparison. Enzymatic and chemical cleavages are typically required steps in order to identify a protein of interest, locate sites of modification (e.g. phosphorylation), understand protein folding (disulfide bonds) etc.