Sample Preparation
***Effective June 28, 2013, the Keck Biotechnology Resource Laboratory will discontinue the following services: Amino Acid Analysis, Protein Sequencing (Edman Degradation), Large- and Small-Scale Peptide Synthesis. Small-Scale Peptide Synthesis is no longer accepting orders. Please contact us for the last dates for order or sample submission related to other services.
Samples may be submitted in solution (or dried from solution), on PVDF membrane, or attached to beads like those used in peptide synthesis resins.
Solution Samples
Glass-fiber filter disks pretreated with ABI Biobrene Plus ™ are used as sample supports for solution samples. We prefer that samples be submitted in small volumes (<50 µl) of volatile solvents that do not contain non-volatile salts or amines.
PVDF Samples
Samples purified by SDS-PAGE must be electroblotted onto PVDF membrane as the polyacrylamide band would dissolve on the sequencer. Nitrocellulose membrane is also not acceptable for the same reason.
Resin Beads
Resin beads used for peptide synthesis (polystyrene) are stable during the Edman chemistry, so peptide or protein samples coupled to them via the C-terminus may be sequenced. (Peptides are synthesized starting at the C-terminus.) Beads should be submitted in 100 µl of water to prevent static making them jump out of the tube when it is opened.
