***Effective June 28, 2013, the Keck Biotechnology Resource Laboratory will discontinue the following services: Amino Acid Analysis, Protein Sequencing (Edman Degradation), Large- and Small-Scale Peptide Synthesis. Small-Scale Peptide Synthesis is no longer accepting orders. Please contact us for the last dates for order or sample submission related to other services.
In the 1980's and early 1990's Edman sequencing was the primary means of identifying proteins. Mass spectrometry of peptides from enzymatic digests is now the most common means of protein identification due to the speed (multiple peptides identified over a 2-3 hour HPLC gradient being fed into an electrospray mass spectrometer vs. 1 amino acid per hour by Edman sequencing) and ability to identify N-teminally blocked proteins and proteins in complex mixtures. However, Edman sequencing can provide data unavailable by mass spectrometric methods and is a powerful complimentary technique.