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W.M. Keck Foundation

Oligo Synthesis Resource
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  • Purification
  • Trityl ON Oligos
  • Modifications
  • Mixed Base/Doped Oligos
  • RNA & 2'-O-Methyl RNA
  • UltraMild Synthesis
  • Protocols
    • Desalting Protocol
    • OPC Purification Protocol
    • Unmodified Trityl-In DNA Oligos
    • Halogenated Trityl-On DNA Oligos
    • 3'ddC Trityl-On DNA Oligos
    • Manual Removal of 5’-Trityl (DMT)
    • Removal of 2'-Protecting Groups (TOM and TBDMS)
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Oligo Synthesis Resource
300 George Street
Room 2128
New Haven, CT 06511
Tel: 203.737.2069
Fax: 203.737.1287
oligos@yale.edu
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Protocols

  • Page Purification Protocol
  • Desalting Protocol using Glen-Pak Cartridges
  • Glen-Pak Purification Protocol for DMT-On Oligos
  • Cleavage and Deprotection of Unmodified Trityl-On DNA Oligos
  • Cleavage and Deprotection of Halogenated Trityl-On DNA Oligos
  • Cleavage and Deprotection of 3'ddC Trityl-On DNA Oligos
  • Manual Removal of 5'-Trityl (DMT) From Trityl-On DNA Oligos Following Deprotection
  • Removal of 2'-Protecting Groups (TOM and TBDMS) from RNA Oligos

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  • Updated 10/18/12