Yale/NIDA Neuroproteomics Center Fact Sheet

Title of Center Grant:Yale/NIDA Neuroproteomics Research Center
Grant Number:2 P30 DA018343
Total Budget Period:8/23/2004 – 5/30/2014
Theme of Center:"Proteomics of Altered Signaling in Addiction"
Principal Investigator:Kenneth R. Williams, Ph.D.
Co-Director, W.M. Keck Foundation
Biotechnology Resource Laboratory
Professor (Adj.) Research
Dept. of Molecular Biophysics & Biochemistry
Yale University School of Medicine
Co-Principal Investigator:Angus C. Nairn, Ph.D.
Professor, Department of Psychiatry
Yale University School of Medicine
Awarding Agency:National Institute on Drug Abuse (NIDA)
National Institutes of Health (NIH)
Center Cores:
  1. Administrative
  2. Bioinformatics and Biostatistics (includes High Performance Computing and Protein Database)
  3. Protein Profiling and Identification, Biophysics, and Phosphoinositide Analysis
  4. Protein Post-Translational Modification Identification and Profiling
  5. Targeted Proteomics
Center Investigators:25 from 10 institutions including Indiana University, Mount Sinai School of Medicine, Rockefeller University, Stanford University, University of Chicago, University of Connecticut Health Center, University of Massachusetts Medical School, University of Texas Southwestern Medical Center, Wake Forest University Medical Center, and Yale University
Specific Aims:
  • Bring together faculty working at the forefront of such key neuroscience areas as signal transduction, plasticity, neuronal morphogenesis, lipid metabolism in neuronal signaling and synaptic function, and response to psychotropic drugs with experts in proteomics, biophysics, biostatistics, bioinformatics, and high performance computing.
  • Apply high-throughput, state-of-the-art proteomic technologies like isobaric tags for relative and absolute quantitation (iTRAQ), LC/MS Label Free Quantitation (LFQ), differential (fluorescence) gel electrophoresis (DIGE), and Multiple Reaction Monitoring (LC-MRM) based targeted proteomics to analyze adaptive changes in neuronal protein expression and regulation that occur in response to drugs of abuse.
  • To advance and develop new separation tools for cellular lipids and neuroproteomic technologies for studying the brain proteome and phosphoproteome and to apply these to studies of the actions of drugs of abuse.
  • Analyze the actions of opiates; the psychostimulants, cocaine and amphetamine; and nicotine on protein expression.
  • Study the proteomic changes that occur both pre- and post-synaptically following the actions of drugs of abuse.
Overall Goal:To substantially increase understanding of the biochemical mechanisms that underlie substance abuse and its treatment.