Primer Sequences

Outlined below are the primer sequences that we have used through the years in our STANDARD reactions. You may want or need to have these custom primers synthesized. They may differ a little from proprietary primers. That does not mean that primers purchased from conventional vendors will not work - we have simply found that this set of primers works well when sequencing with a wide array of vectors.

Primer

Sequence (5'-3')

M13-21

TGT AAA ACG ACG GCC AGT

M13-47

CGC CAG GGT TTT CCC AGT CAC GAC

M13-REV4

TCA CAC AGG AAA CAG CTA TGA C

T7

TAA TAC GAC TCA CTA TAG GG

T7TERM

GCT AGT TAT TGC TCA GCG G

T3

ATT AAC CCT CAC TAA AGG GA

PCRIIT7

CGA CTC ACT ATA GGG CGA ATT GGG

PCRIISP6

GGT GAC ACT ATA GAA TAC TCA AGC

SP6

GAT TTA GGT GAC ACT ATA G

poly TG

(T)25G

poly TC

(T)25C

poly TA

(T)25A

SK

TCT AGA ACT AGT GGA TC

KS

CGA GGT CGA CGG TAT CG

Primers may be ordered via the Keck Biotechnology Resource Lab.