High Volume Submission Policies

High Volume Submission Policies

The high volume, 96 well plate format is available for researchers who are experienced users of the facility and are confident in their methodology for template and primer preparation. We sequence and charge for all samples submitted on the plate regardless of the results, unless it is a clear technical error on our part.

  • All high volume plate submissions must be in one of the following plates and sealed carefully with strip caps or sealing foil:
  • 96-well Eppendorf twin tec plates Part#951020389,951020346, 951020320, 951020362(Strip caps Part# 951023035)
    • Applied Biosystems Optical 96-well reaction plates Part# N801-0560 (Strip Caps Part# N801-0534)

 

**The plates are located in the Yale stockrooms or they can be ordered on SciQuest**

    • DOT Scientific PCR plates Part # 351-PCR and 352-PCR (Strip Caps Part#405-12PCR)
**Contact Matthew Steinmetz for pricing: 

msteinmetz@dotscientific.com

**Sealing foil can be purchased from DOT Scientific, Part #T392.

Contact Matthew for pricing.

The minimum number of samples for a single high volume plate is 48. When submitting less than 96 samples on a plate, please submit in multiples of 12- placing the samples in rows of 12 consecutively i.e. begin at A1 through A12 then B1-B12, C1-12, etc.

  • High volume plates should be dropped off with a high volume submission confirmation sheet (available after web-ordering via our “Sample Submission” page).

High Volume Plate Submission Protocol

High Volume 96-well reaction

Add to each well:

2 µl of 4 µM custom or universal primer

To each well, add:
  • 600 ng plasmid DNA or
  • 10-20 ng per 200bp of PCR product (e.g. for a 400bp PCR product each well should contain 20-40 ng DNA) or
  • 2000 ng cosmid or lambda DNA or
  • 4000 ng BAC DNA
  • Dilute your product to 16µL with H2O

Therefore, each well should include:

16 µL including your product + H2O
  2 µL4µM primer     
18 µL total volume per well

Cap the plate firmly using the strip caps or sealing film and wrap each plate with parafilm.(Sealing film is preferable to strip caps.)

Be sure to label each plate with a unique name. Results will be labeled with the researcher code -plate name -and well-coordinate (RES CODE-plate name-A1-12, RES CODE-plate name-B1-12 etc). The researcher is responsible for holding the "key" to the well co-ordinates for sample identification.

Standard Turnaround Time

Our turnaround time for a single plate is 8-24 hours from the time we receive your plate (except for plates received on Friday). Please allow extra time when you submit more than one plate at a time.

Seq Protocols

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Primer Guidelines

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Primer Sequences

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Template Preparation

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Individual Tube Submission Policies and Procedures

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High Volume Plate Sequencing

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Direct Sequencing of PCR Products

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PCR Purification

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Fragment Analysis