Stopped-Flow Spectrofluorometer

The facility houses an Applied Photophysics SX.18MV Stopped-Flow instrument that has recently been upgraded to operate with the latest Pro-Data windows software used on the current model SX20 stopped-flows and also the Chirascan Circular Dichroism instrument.

The SX is a state-of-the-art stopped-flow reaction analysis system featuring absorbance and fluorescence detection. The system is equipped with a low-volume stopped-flow cell in which microliter volumes of sample are mixed and resulting signal changes can be monitored on a time scale from milliseconds to 1000s.

In addition to the standard single mix experiment where two reagents are mixed, the SX.18MV is equipped with the sequential-mixing ('double-mixing') accessory. In this mode, two reagents are mixed in an aging loop and after a user-defined ageing time mixed with a third reagent.

SX.18MV Stopped-Flow Reaction Analyzer

  • optimized for absorbance and fluorescence acquisition
  • automated sample handling unit
  • millisecond dead-time enabling measurement of rate constants of up to 1500 s-1
  • minimum volume requirement: 40 microliters of reagent from each syringe
  • variable temperature capabilities
For further information about the SX series of stopped-flow instruments visit the Applied Photophysics website at www.photophysics.com

Contact Information:

Jamie Towle-Weicksel
Hunter Radiation Therapy
15 York Street (Room WWW 308)
New Haven, CT 06510
Phone: 203-785-4441 
email: jamie.towle-weicksel@yale.edu

Daria Sizova 
Hunter Radiation Therapy
15 York Street (Room WWW 308B)
New Haven, CT 06510
Phone: 203-785-4441
email: daria.sizova@yale.edu