Correlation between the amount of protein analyzed and the accuracy of MW determination using DAWN® DSP and OPTILAB DSP detectors.

Look at the actual data in the "micro analyses" graph


Protein
Amount loaded
[micrograms]
number of runs
Pred. MW
[kDa] a
Average experimental MW
[kDa]
Range of
experimental accuracy
[%]b
Standard deviation
[kDa]
Ovalbumin 150 4 42.8 42.4 0.2 to 1.6 0.3
100 7 42.8 42.3 0.2 to 2.4 0.8
45-50 4 42.8 41.6 0.5 to 5.8 1
6-10 5 42.8 42.9 1.4 to 4.5 1.5
Transferrin 100 3 75.2 76.5 0.7 to 3.2 1
8 5 75.2 76.3 0.3 to 5.2 2

(column: TSK GEL G3000SWXL [TosoHaas], buffer: 20 mM phosphate, 150 mM NaCl, pH=7.5).


a predicated MW was calculated based on protein sequence as retrieved from the ExPASy molecular biology WWW server of the Swiss Institute of Bioinformatics (SIB). http://expasy.hcuge.ch/

b Experimental accuracy (%) is calculated as an absolute value of {100*[(Average Experimental MW-predicted MW)/predicted MW]}