Correlation between the amount of protein analyzed and the accuracy of MW determination using DAWN® DSP and OPTILAB DSP detectors.

Look at the actual data in the "micro analyses" graph


Protein
Amount loaded
[micrograms]
number of runs
Pred. MW
[kDa] a
Average experimental MW
[kDa]
Range of
experimental accuracy
[%]b
Standard deviation
[kDa]
Ovalbumin150442.842.40.2 to 1.60.3
100742.842.30.2 to 2.40.8
45-50442.841.60.5 to 5.81
6-10542.842.91.4 to 4.51.5
Transferrin100375.276.50.7 to 3.21
8575.276.30.3 to 5.22

(column: TSK GEL G3000SWXL [TosoHaas], buffer: 20 mM phosphate, 150 mM NaCl, pH=7.5).


a predicated MW was calculated based on protein sequence as retrieved from the ExPASy molecular biology WWW server of the Swiss Institute of Bioinformatics (SIB). http://expasy.hcuge.ch/

b Experimental accuracy (%) is calculated as an absolute value of {100*[(Average Experimental MW-predicted MW)/predicted MW]}