Nadia Annitia Ameen MBBS
Associate Professor of Pediatrics (Gastroenterology) and of Cellular and Molecular Physiology
Apical endocytosis and exocytosis; Protein traffic; Secretory diarrhea; Cystic fibrosis
My laboratory has been supported continuously by NIH awards (K08, R03, R01) to study intracellular trafficking routes and mechanisms and that regulate the expression and function of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel in the intestine. CFTR represents the primary exit pathway responsible for anion and fluid secretion on the apical membranes of intestinal cells. Mutations in the CFTR gene result in absence of functional CFTR channels and the genetic disease Cystic Fibrosis while up-regulation of CFTR function is implicated in the pathogenesis of diarrheal disease. We employ transgenic animal and polarized intestinal cell models in conjunction with cell biologic, molecular and physiologic approaches to understand the intracellular trafficking routes traversed by CFTR and how alterations in these pathways lead to intestinal diseases.