Gary W Cline PhD
Associate Professor of Medicine (Endocrinology); Director, Analytical Core, Mouse Metabolic Phenotyping Center at Yale Univ. School of Medicine
Diabetes mellitus has a profound impact on hepatic and peripheral
glucose metabolic fluxes. My studies on the biochemical mechanisms
responsible for the pathophysiology of diabetes mellitus primarily
employ nuclear magnetic resonance spectroscopy, mass spectroscopy, and
stable isotopic techniques. Using these techniques, the flux and fate
of key intermediates of carbohydrate and lipid metabolism can be
determined in vitro and in vivo. These techniques are developed in cell
systems and small animal models and when appropriate applied to studies
of human physiology. New areas of research are the application and
adaptation of these techniques to the study of pancreatic beta-cell
metabolism and glucose-stimulated insulin release.
Recent dramatic improvement in the success rate of islet transplantation in humans has prompted interest for more widespread application of this methodology. My future research will explore the development of NMR based methods for non-invasive in vitro quality assessment of tissue destined for transplantation, and in vivo monitoring of islet transplants.