Yale Core Center for Musculoskeletal Disorders

The Yale Core Center for Musculoskeletal Disorders is dedicated to fostering research in disorders of skeletal tissue and muscle, with the ultimate goal of improving human health. The Center is particularly interested in supporting the development and comprehensive investigation of animal models of musculoskeletal disorders. Investigators whose interests include cellular models of disease, the investigation of novel physiologic pathways or therapeutic interventions are also welcome.

The Center consists of 4 Cores:

The Administrative Core
   Pilot and Feasibility Program
   Education and Seminar Program

The Molecular Core
   Construction of transgenes and gene disruption vectors
   Identification of genetically altered or naturally occurring mutant mice
   Localization of gene expression by in situ hybridization
   Real-time PCR
   Quantitation of gene expression
   Technical assistance for both basic and advanced methods in molecular biology

The Physiology Core
   Preparation of undecalcified tissue sections and specialized staining
   Interpretation of static and dynamic parameters of bone histomorphometric analysis
   Measurement of biochemical markers of bone turnover and measurement of bone cell markers
   Assessment of bone mineral density ex vivio using peripheral quantitative computed tomography
   MicroCT imaging
   Instruction to technical staff, fellows, and junior faculty

The Cell Core
   Isolation and cultivation of primary murine and fetal rat calvarial osteoblasts
   Cultivation of murine osteoclast-like cells generated from in vitro co-culture
   Training in the in vitro isolation and culture of osteoblastic and osteoclastic cells

Analysis of Skeletal Tissues: State of the Art 2007

We put together a short workshop on current methods of analysis for skeletal tissue. Many of these methods are not well understood, and yet they are the basic investigative tools of our trade.