CyTOF 2: Time-of-Flight mass spectrometer for high-speed acquisition of highly multi-parametric single cell data

The CyTOF 2 analyzes cells labeled with stable heavy metal isotopes using time-of-flight atomic mass cytometry technology.

  • Multi-parametric single cell data, deep profiling 
  • Detect up to 34 parameters simultaneously in a single tube (with theoretical limit of 100 parameters) 
  • Detect more from limited samples 
  • Advantages over traditional flow cytometry: No background, no overlap between channels, no compensation issues 
  • The CyTOF is checked daily for optimal performance. Ala Nassar is available during work hours for consultation

Reagents: Labeled antibodies are available from DVS Sciences (DVSSciences.com) including metal-conjugated individual antibodies (>200); ready to use MaxPar Panel Kits comprised of 6-17 well-characterized markers for human or mouse cell populations; and metal labeling reagents for in-house conjugation of antibodies. Note that metal conjugated anti-fluorochrome antibodies (e.g. anti-FITC) are also available that can be used with existing fluorescent reagents. 

Biosafety Policies: All infectious, human, and non-human primate cells must be fixed before they are analyzed on the CyTOF. 

Charges: Analysis is available as a fee for service and usage with Ala Nassar or by independent users after completing training. Non-Yale users will be accommodated as possible. Please contact Dr. Montgomery.

Sample Submission Form
Please submit completed Sample Submission Form to Dr. Ala Nassar.
Contact

Director:
Ruth R. Montgomery
, PhD (ruth.montgomery@yale.edu

Biotechnology Associate:
Ala Nassar, PhD
(ala.nassar@yale.edu

Location: TAC S416
Phone: (203) 785-7049