Scott Swenson MD, PhD
Assistant Professor of Medicine (Digestive Diseases); Associate Director, Fellowship Training Program in Digestive Diseases
Stem and progenitor cells; Liver; Liver injury; Pancreatic acinar cells; Exocrine function; Secretion
I am investigating the role of stem and progenitor cells in the liver during normal development and in response to liver injury. The liver's remarkable capacity for regeneration after injury suggests that a better understanding of these phenomena will lead to novel cell therapy and regenerative medicine approaches to acute and chronic liver disease. Under some conditions, cells arising from the bone marrow may also contribute to liver regeneration.
The Y chromosome (pink dots) colocalizes with HNF-1 (green nuclei). Nuclei are counterstained with DAPI (blue). The recipient mouse had a homozygous targeted mutation in the Fumaryl acetoacetate hydrolase (FAH) gene, which models the human liver disease, Hereditary Tyrosinemia. Transplantation of normal bone marrow results in extensive repopulation of normal hepatocytes in the tyrosinemic liver. The presence of two or more Y chromosomes in some hepatocytes is consistent with the normal phenomenon of hepatocyte polyploidy.
Ongoing studies will address the issue of whether stable in vivo fusion of cells of hematopoietic origin, such as Kupffer cells, with injured hepatocytes always accounts for the apparent developmental "plasticity" of bone marrow-derived cells.
- Direct conversion of mouse fibroblasts to hepatocyte-like cells using forced expression of endodermal transcription factors (editorial). ES Swenson. Hepatology 2011 (in press).
- The blood vessels of Prometheus (editorial). ES Swenson and DS Krause. The Hematologist 2011;8:8.
- Stem cell therapeutics: potential in the treatment of inflammatory bowel disease. ES Swenson, ND Theise. Clin Exp Gastro 2010;3:1-10.
- Adenosine inhibits bone marrow mesenchymal stem cell chemotaxis by down-regulation of Rac1. M Mohamadnejad, MA Sohail, A Watanabe, DS Krause, ES Swenson, WZ Mehal. Hepatology 2010;51:963-73. PMID 20044808, PMC2840188.
- Hepatocyte nuclear factor-1 as marker of hepatic epithelial phenotype reveals bone marrow-derived hepatocytes, but not oval cells, after liver injury in mice (2008). ES Swenson, I Guest, Z Ilic, M Mazzeo, P Lizardi, C Hardiman, S Sell, DS Krause. Stem Cells 26:1768-1777.
- The hepatic stem cell niche: identification by label retaining cell assay. (2008). R Kuwahara, AV Kofman, CS Landis, ES Swenson, E Barendswaard, ND Theise ND. Hepatology 47:1994-2002.
- Physiologic variations in stem cell factor and stromal-derived factor-1 in murine models of liver injury and regeneration (2007). ES Swenson, R Kuwahara, DS Krause, ND Theise. Liver International 28: 308-318.
- Induced pluripotent cells mimicking human embryonic stem cells. M Mohamadnejad M, ES Swenson (2008). Arch Iran Med. 11:125-8.
- Limitations of green fluorescent protein (GFP) as a cell lineage marker (2007). ES Swenson, JG Price, T Brazelton, DS Krause. Stem Cells 25: 2593-2600.
- Induced pluripotent cells mimicking human embryonic stem cells. M Mohamadnejad M, ES Swenson (2008). Arch Iran Med. 11: 125-8.